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AB278114

Human L1CAM ELISA Kit

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Human L1CAM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human L1CAM with a sensitivity of 17.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

4 이미지
Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)
  • sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Interpolated concentrations of native L1CAM in human serum and plasma.

The concentrations of L1CAM were measured in duplicates, interpolated from the L1CAM standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 17.32 ng/mL in serum, 14.92 ng/mL in plasma (citrate), 15.41 ng/mL in plasma (EDTA), and 14.45 ng/mL plasma (heparin).

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)
  • sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Interpolated concentrations of native L1CAM in HeLa cell extract based on a 50 μg/mL extract load.

The concentrations of L1CAM were measured in duplicate and interpolated from the L1CAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 6.83 ng/mL in HeLa extract.

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)
  • sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

12.5% Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 17.90 ng/mL with a range of 13.70 – 25.20 ng/mL.

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)
  • sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Example of human L1CAM standard curve in Sample Diluent NS.

The L1CAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 17.2 pg/mL

검출 범위

101.56 - 6500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Assay Kits

AB315307

Human NCAM1 ELISA Kit

SimpleStep

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

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Proteins & Peptides

AB114149

Recombinant Human GFAP protein (GST tag N-Terminus)

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

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Primary Antibodies

AB237456

Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] (AB237456)

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  • 0
Sample Prep & Detection Kits

AB269820

Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link®

Conjugation - Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link® (AB269820)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human L1CAM ELISA kit (ab278114) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human L1CAM protein in human serum, plasma, cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human L1CAM with 17.20 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Neural cell adhesion molecule L1 (L1CAM) is involved in dynamics of cell adhesion and generation of transmembrane signals at tyrosine kinase receptors. Studies suggest L1CAM functions during human brain development. L1CAM is critical neuronal migration, axonal growth and fasciculation, and synaptogenesis processes. In the mature brain, L1CAM plays a role in the dynamics of neuronal structure and function, including synaptic plasticity. Human L1CAM shows 88%, 88%, 99% and 89% sequence homology with mouse, rat, monkey, and cow, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 97 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "89 - 99 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "92 - 110 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "85 - 104 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "90 - 99 %", "average": "= 94" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278114-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB278114-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278114-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278114-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278114-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278114-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278114-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278114-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB278114-CMP13", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB278114-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human L1CAM Lyophilized Recombinant Protein", "number":"AB278114-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human L1CAM Capture Antibody", "number":"AB278114-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human L1CAM Detector Antibody", "number":"AB278114-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
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제품 프로토콜

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타겟 정보

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
See full target information L1CAM
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 9:eadi3647 PubMed37713494

2023

ATP1A3 as a target for isolating neuron-specific extracellular vesicles from human brain and biofluids.

Applications

Unspecified application

Species

Unspecified reactive species

Yang You,Zhengrong Zhang,Nadia Sultana,Maria Ericsson,Yuka A Martens,Min Sun,Takahisa Kanekiyo,Seiko Ikezu,Scott A Shaffer,Tsuneya Ikezu
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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