Human LAMC2 ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)
Interpolated concentrations of native LAMC2 in human cell culture supernatant samples.
The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 supernatant 1 : 100 and A549 supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 86,513 pg/mL in A431 supernatant, 3,106 pg/mL in A549 supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)
Example of human LAMC2 standard curve in Sample Diluent NS.
The LAMC2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human LAMC2 ELISA Kit (AB282301)
Interpolated concentrations of native LAMC2 in human serum, plasma samples.
The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) neat, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 368.1 pg/mL in serum, 366.7 pg/mL in plasma (citrate) and 274.7 pg/mL in plasma (EDTA), and 463.9 pg/mL plasma (heparin).
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제품 세부 정보
Human LAMC2 ELISA kit (ab282301) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LAMC2 protein in human serum, plasma, cell culture supernatant, and cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LAMC2 with 2.16 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
LAMC2 is the gamma subunit of Laminin 5. Laminin's are complex glycoproteins consisting of an alpha, beta and gamma chain. LAMC2 secreted in lung cancer and associated with the progression of the disease. ab282301 recognizes a fragment of the gamma-2 subunit.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LAMC2 plays a critical role in cell adhesion migration and signal transduction. Laminin-332 which includes LAMC2 binds to integrin receptors on cell surfaces facilitating cell-matrix interactions and communication. This complex is important for basement membrane stability and tissue organization and it is actively involved in wound healing by promoting keratinocyte migration and melanin transport.
Pathways
LAMC2 integrates into several important extracellular matrix pathways including the integrin signaling pathway. These pathways involve interactions with proteins like integrin alpha-6/beta-4 which are linked to cellular adhesion and mobility. LAMC2 also contributes to epithelial cell functions and is involved in the PI3K/AKT signaling pathway highlighting its importance in cell proliferation and survival.
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NPJ precision oncology 8:250 PubMed39496753
2024
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