AB288588

Human Laminin Beta 2 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Human Laminin Beta 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Laminin Beta 2 with a sensitivity of 2.498 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

ELISA

Supplier Data

ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

sELISA

Supplier Data

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 25% HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Interpolated concentration of native Laminin Beta 2 was measured in duplicate at different sample concentrations. Undiluted samples are 5% serum, plasma (heparin), plasma (EDTA), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Example of human Laminin Beta 2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.498 pg/mL

검출 범위

6.25 - 400 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Laminin Beta 2 ELISA Kit ab288588 is a rapid single-wash 90-min Sandwich ELISA to measure Human Laminin Beta 2 in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.498 pg/mL.

How the assay works

Human Laminin Beta 2 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Laminin Beta 2 ELISA Kit ab288588 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Laminin beta 2 antibody [EPR23569-8] - BSA and Azide free (Capture) ab281224
- Anti-Laminin beta 2 antibody [EPR23569-82] - BSA and Azide free (Detector) ab281074

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 113 %", "average": "= 103" }, { "sample": "Serum", "range": "105 - 107 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "102 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "101 - 115 %", "average": "= 110" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288588-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB288588-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB288588-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288588-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288588-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288588-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288588-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288588-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Laminin Beta 2 Capture Antibody", "number":"AB288588-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Laminin Beta 2 Lyophilized Recombinant Protein", "number":"AB288588-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Laminin Beta 2 Detector Antibody", "number":"AB288588-CMP05", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Laminin beta 2 also known as laminin B2 or laminin beta chain is an important component of the extracellular matrix. This laminin molecule has a molecular weight of approximately 210 kDa. It forms an essential part of the structural integrity in tissues by serving as a basement membrane protein. Expression of laminin beta 2 occurs extensively in the kidney peripheral nerves and neuromuscular junctions. It plays a role in binding other laminin subunits to form functional heterotrimeric laminin molecules.

Biological function summary

Laminin beta 2 is involved in the formation and maintenance of the basement membrane structure contributing to tissue cohesion and cell adhesion. It is part of the laminin-521 complex which involves two other chains that together foster cellular adhesion migration and differentiation. This laminin molecule supports developmental processes by providing signaling cues that influence the behavior of various cell types making it indispensable in organ development and repair.

Pathways

Laminin beta 2 interacts within the integrin signaling pathway where it mediates cell adhesion and communication. It also plays an important role in the basement membrane assembly pathway interacting closely with integrin α3β1. Through these pathways laminin beta 2 maintains cellular organization and stability reflecting its integrative role together with related proteins like nidogen and collagen IV.

Laminin beta 2 mutations link to Pierson syndrome a condition characterized by kidney and eye abnormalities. Additionally abnormalities in laminin beta 2 expression have associations with congenital muscular dystrophy where the integrity of neuromuscular junctions is compromised. Genetic studies connect it with the dystroglycan protein highlighting its significance in maintaining tissue architecture and function in the context of these disorders.

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제품 프로토콜

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타겟 정보

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB2

대체 명칭 보기

LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of biological sciences 20:5531-5547 PubMed39494341

2024

Reconstruction of the Hepatic Microenvironment and Pathological Changes Underlying Type II Diabetes through Single-Cell RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Yen Dai,Ying-Ming Tsai,Chao-Yuan Chang,Hung-Pei Tsai,Kuan-Li Wu,Yu-Yuan Wu,Ling-Yu Wu,Shu-Fang Jian,Pei-Hsun Tsai,Chai-Tung Ong,Chien-Hui Sun,Ya-Ling Hsu

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Laminin Beta 2 ELISA Kit

ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

Sandwich ELISA - Human Laminin Beta 2 ELISA Kit (AB288588)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise