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AB327308

Human LECT2 ELISA Kit, Chemiluminescent

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Human LECT2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human LECT2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.14 - 300 ng/ml with a sensitivity of 0.07 ng/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)
  • sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Serum from ten individual healthy human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean LECT2 concentration was determined to be 51 ng/mL with a range of 20 - 125 ng/mL.

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)
  • sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Interpolated concentrations of native LECT2 in human lung extract based on a 1000 µg/mL extract load.

The concentrations of LECT2 were measured in duplicate and interpolated from the LECT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean LECT2 concentration was determined to be 8.8 ng/mL in lung extract.

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)
  • sELISA

Supplier Data

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Interpolated concentrations of native LECT2 in human serum and plasma samples.

The concentrations of LECT2 were measured in duplicates interpolated from the LECT2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% plasma-citrate 25% plasma-EDTA 25% and plasma-heparin 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean LECT2 concentration was determined to be 28.4 ng/mL in serum 22.5 ng/mL in plasma-citrate 32.5 ng/mL in plasma-EDTA and 13.0 ng/mL in plasma-heparin.

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)
  • sELISA

Lab

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Standard curve comparison between the Chemiluminescent Human LECT2 SimpleStep ELISA and colorimetric Human LECT2 SimpleStep ELISA (ab269554) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)
  • sELISA

Lab

Sandwich ELISA - Human LECT2 ELISA Kit, Chemiluminescent (AB327308)

Dynamic range comparison between the Chemiluminescent Human LECT2 SimpleStep ELISA, colorimetric Human LECT2 SimpleStep ELISA (ab269554), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, EDTA Plasma, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human, Monkey

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.07 ng/mL

검출 범위

0.14 - 300 ng/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human LECT2 ELISA Kit, Chemiluminescent (ab327308) is a rapid single-wash sandwich 55-min ELISA to measure Human LECT2 in Serum, EDTA Plasma, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.07 ng/mL.

How the assay works

Human LECT2 ELISA Kit, Chemiluminescent (ab327308) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LECT2 (ab327308) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Human LECT2 antibody [EPR22049-227] - BSA and Aside free (Capture) ab270338
- Anti-Human LECT2 antibody [EPR22049-87] - BSA and Aside free (Detector) ab270339

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 96 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "113 - 118 %", "average": "= 115" }, { "sample": "Tissue Extracts", "range": "120 - 120 %", "average": "= 120" }, { "sample": "Heparin Plasma", "range": "99 - 103 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "111 - 117 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "81 - 85 %", "average": "= 83" } ]
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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327308-10x96Tests", "assetComponentDetails": [ { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327308-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327308-CMP04" }, { "size":"10 x 600 µL", "name":"10X Human LECT2 Detector Antibody", "number":"AB327308-CMP02" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327308-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327308-CMP11" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327308-CMP06" }, { "size":"10 x 600 µL", "name":"10X Human LECT2 Capture Antibody", "number":"AB327308-CMP01" }, { "size":"10 x 1 Vial", "name":"Human LECT2 Lyophilized Recombinant Protein", "number":"AB327308-CMP03" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327308-CMP10" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327308-CMP09" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327308-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327308-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327308-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327308-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LECT2 Detector Antibody", "number":"AB327308-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327308-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327308-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327308-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327308-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327308-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327308-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LECT2 Lyophilized Recombinant Protein", "number":"AB327308-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LECT2 Capture Antibody", "number":"AB327308-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation (PubMed : 9524238). Does not show metalloendopeptidase activity (PubMed : 27334921).
See full target information Leukocyte cell-derived chemotaxin-2

대체 명칭 보기

Leukocyte cell-derived chemotaxin-2, LECT-2, hLECT2, LECT2
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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