JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB303746

Human LHB ELISA Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human LHB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LHB with a sensitivity of 21.227 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 이미지
Sandwich ELISA - Human LHB ELISA Kit (AB303746)
  • sELISA

Supplier Data

Sandwich ELISA - Human LHB ELISA Kit (AB303746)

Interpolated concentrations of LHB in normal human serum donors

Serum of 4 individual healthy human female donors (donors 1-4) and 4 individual healthy human male donors (donors 5-8) was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LHB concentration was determined to be 4985 pg/mL with a range of 717 - 14998 pg/mL for female donors. The mean LHB concentration was determined to be 651 pg/mL with a range of 403 - 936 pg/mL for male donors.

Sandwich ELISA - Human LHB ELISA Kit (AB303746)
  • sELISA

Supplier Data

Sandwich ELISA - Human LHB ELISA Kit (AB303746)

Interpolated concentrations of human LHB in serum, plasma (EDTA), plasma (citrate), plasma (heparin), and urine.

Interpolated concentration of native LHB was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, plasma (heparin) 25%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human LHB ELISA Kit (AB303746)
  • sELISA

Supplier Data

Sandwich ELISA - Human LHB ELISA Kit (AB303746)

Example of human LHB standard curve in Sample Diluent NS.

Example of human LHB standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 21.227 pg/mL

검출 범위

42.969 - 2750 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB100533

Human hCG ELISA Kit

Sandwich ELISA - Human hCG ELISA Kit (AB100533)

  • 0
  • 0
Assay Kits

AB108666

Testosterone ELISA Kit

Competitive ELISA - Testosterone ELISA Kit (AB108666)

  • 4
  • 5
Assay Kits

AB108667

17 beta Estradiol ELISA Kit

Competitive ELISA - 17 beta Estradiol ELISA Kit (AB108667)

  • 4
  • 2
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human LHB SimpleStep ELISA® kit (ab303746) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LHB protein in serum, plasma (EDTA), plasma (hep), plasma (cit), urine and cell culture media. Quantitate Human LHB with 21.227 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
style
left-column

Recovery

[ { "sample": "Cell culture media", "range": "108 - 115 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "80 - 101 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "83 - 104 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "89 - 94 %", "average": "= 92" }, { "sample": "Serum", "range": "92 - 119 %", "average": "= 105" }, { "sample": "Urine", "range": "84 - 89 %", "average": "= 86" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303746-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB303746-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB303746-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303746-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303746-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303746-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303746-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303746-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LHB Capture Antibody", "number":"AB303746-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LHB Lyophilized Recombinant Protein", "number":"AB303746-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LHB Detector Antibody", "number":"AB303746-CMP03", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LHB also known as luteinizing hormone beta or beta LH is a subunit of luteinizing hormone (LH). It plays an important mechanical role in gonadal function. LHB shares a common alpha subunit with other glycoprotein hormones while the beta subunit which is unique to each hormone confers biological specificity. LHB contributes to the dimeric structure of LH giving it a molecular mass around 30 kDa. This protein is expressed mainly in the anterior pituitary gland where it interacts with cells that influence reproductive processes.
Biological function summary

LHB binds with the alpha subunit to form the active LH hormone which importantly stimulates the ovaries in females and testes in males. In females LH promotes the production of estrogen and progesterone facilitating ovulation and menstrual cycles. In males it stimulates Leydig cells leading to testosterone production. LH hormone functions as part of a complex with its receptor a G protein-coupled complex influencing the reproductive system's normal operations.

Pathways

The LHB is central to the hypothalamic-pituitary-gonadal axis a feedback loop that regulates reproductive physiology. Within this axis gonadotropin-releasing hormone (GnRH) from the hypothalamus stimulates LHB production in concert with follicle-stimulating hormone (FSH). These hormones together regulate the development and function of the gonads. LHB's interaction with LH receptors on gonadal tissues signifies its operational involvement in the steroidogenesis and gametogenesis pathways.

Abnormalities in LHB expression associate with conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS altered LHB levels lead to hormonal imbalances affecting fertility. Hypogonadism may result from insufficient LHB affecting sexual development and function. LHB's influence on testosterone and estrogen levels links it closely with disorders characterized by hormonal deficiencies or excesses tying it to complications involving the HPG axis.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
See full target information LHB

대체 명칭 보기

Lutropin subunit beta, Lutropin beta chain, Luteinizing hormone subunit beta, LH-B, LSH-B, LSH-beta, LHB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com