AB242228

Human LIF ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Human LIF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LIF with a sensitivity of 1.469 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

6 이미지

ELISA

Supplier Data

ELISA - Human LIF ELISA Kit (AB242228)

sELISA

Supplier Data

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Human LIF Standard Curve Comparison.

Standard Curve comparison between human LIF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Unknown

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Interpolated concentrations of native LIF in human cell culture supernatant samples. The concentrations of LIF were measured in duplicates, interpolated from the LIF standard curves and corrected for sample dilution. Undiluted samples are 1.5% PHA-M treated PBMC cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIF concentration was determined to be 8336.44 pg/mL in 1.5% PHA-M treated PBMC cell culture supernatant.

sELISA

Unknown

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Example of human LIF standard curve in Sample Diluent NS. The LIF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Example of human LIF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Interpolated concentration of native LIF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 16 stimulated human PBMC supernatant (1.5% PHA-M for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.469 pg/mL

검출 범위

23.44 - 1500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human LIF ELISA Kit ab242228 is a rapid single-wash 90-min Sandwich ELISA to measure Human LIF in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.469 pg/mL.

How the assay works

Human LIF SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human LIF ELISA Kit ab242228 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-LIF antibody [EPR21949-30] - BSA and Azide free (Capture) ab259460
- Anti-LIF antibody [EPR21949-92] - BSA and Azide free (Detector) ab259460

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Cell Media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 111 %", "average": "= 102" }, { "sample": "Serum", "range": "85 - 93 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "87 - 92 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "87 - 92 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "89 - 91 %", "average": "= 89" } ]

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제품 구성

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab242228-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB242228-CMP09" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB242228-CMP12" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB242228-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB242228-CMP05" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB242228-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242228-CMP10" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242228-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human LIF Detector Antibody", "number":"AB242228-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human LIF Capture Antibody", "number":"AB242228-CMP06" }, { "size":"2 x 1 Vial", "name":"Human LIF Lyophilized Recombinant Protein", "number":"AB242228-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242228-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB242228-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB242228-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242228-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242228-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242228-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242228-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242228-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LIF Lyophilized Recombinant Protein", "number":"AB242228-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LIF Capture Antibody", "number":"AB242228-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LIF Detector Antibody", "number":"AB242228-CMP07", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Leukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.

Biological function summary

LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.

Pathways

The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.

LIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.

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제품 프로토콜

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타겟 정보

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

See full target information LIF

대체 명칭 보기

HILDA, LIF, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:360 PubMed40319033

2025

Comprehensive secretome profiling and CRISPR screen identifies SFRP1 as a key inhibitor of epidermal progenitor proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Cheng,Shaohong Isaac Peng,Yunlong Y Jia,Elton Tong,Scott X Atwood,Bryan K Sun

Cell death & disease 15:218 PubMed38490994

2024

TGF-β-driven LIF expression influences neutrophil extracellular traps (NETs) and contributes to peritoneal metastasis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fangbin Zhang,Yan Yan,Xinguang Cao,Changqing Guo,Ke Wang,Shuai Lv

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human LIF ELISA Kit

ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

Sandwich ELISA - Human LIF ELISA Kit (AB242228)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise