JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB215541

Human Lipocalin-2 ELISA Kit

Be the first to review this product! 리뷰 제출

|

(3 제품이 사용된 논문 )

Human Lipocalin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lipocalin-2 with a sensitivity of 14.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
7 이미지
ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • ELISA

Supplier Data

ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Example of human Lipocalin-2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Interpolated concentrations of native Lipocalin-2 in human serum and plasma samples.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 2%, plasma (EDTA) 2%, and plasma (heparin) 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 61.4 ng/mL in serum, 52.2 ng/mL in plasma (citrate), 85.5ng/mL in plasma (EDTA) and 52.4ng/mL in plasma (heparin).

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Interpolated concentrations of native Lipocalin-2 in human urine and saliva samples.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 3% and saliva 0.05%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 67.9 ng/mL in urine and 1,551ng/mL in saliva.

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • sELISA

Unknown

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Human Lipocalin-2 standard curve comparison data.

Standard curve comparison between human Lipocalin-2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 77 ng/mL with a range of 37 – 135 ng/mL.

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lipocalin-2 ELISA Kit (AB215541)

Interpolated concentrations of native Lipocalin-2 in human A431 cell culture supernatant and peripheral blood monocyte (PBMC) cell culture supernatant.

The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 2% and PBMC 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 65.8 ng/mL in A431 and 4.85 ng/mL in PBMC cell culture supernatant.

주요 정보

검출 방식

Colorimetric

샘플 타입

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 14.8 pg/mL

검출 범위

46.9 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB213477

Mouse TIM 1 ELISA Kit (KIM-1)

Recombinant|SimpleStep

ELISA - Mouse TIM 1 ELISA Kit (KIM-1) (AB213477)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB263885

Mouse Calprotectin ELISA Kit (S100A8/S100A9)

Recombinant|SimpleStep

ELISA - Mouse Calprotectin ELISA Kit (S100A8/S100A9) (AB263885)

  • 5
  • 2
Assay Kits

AB201280

Mouse Cystatin C ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Cystatin C ELISA Kit (AB201280)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human Lipocalin-2 ELISA Kit ab215541 is a rapid single-wash 90-min Sandwich ELISA to measure Human Lipocalin-2 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 14.8 pg/mL.

How the assay works

Human Lipocalin-2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Lipocalin-2 ELISA Kit ab215541 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity and renal development. Mice deficient in Lipocalin-2 appear normal but have increased susceptibility to bacterial infection. The bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Human Lipocalin-2 has 62% protein sequence identity to mouse Lipocalin-2.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma EDTA", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "Plasma EDTA", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 106 %", "average": "= 104" }, { "sample": "Serum", "range": "104 - 108 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "107 - 111 %", "average": "= 109" }, { "sample": "Saliva", "range": "107 - 111 %", "average": "= 109" }, { "sample": "Urine", "range": "102 - 118 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "99 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "104 - 109 %", "average": "= 106" } ]
style
left-column

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab215541-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB215541-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB215541-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215541-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB215541-CMP07" }, { "size":"1 x 6000 µL", "name":"10X Human Lipocalin-2 Capture Antibody", "number":"AB215541-CMP05" }, { "size":"2 x 10 Vial", "name":"Human Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB215541-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB215541-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215541-CMP02" }, { "size":"1 x 6000 µL", "name":"10X Human Lipocalin-2 Detector Antibody", "number":"AB215541-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215541-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215541-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB215541-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB215541-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215541-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215541-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215541-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215541-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215541-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Lipocalin-2 Lyophilized Recombinant Protein", "number":"AB215541-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lipocalin-2 Capture Antibody", "number":"AB215541-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lipocalin-2 Detector Antibody", "number":"AB215541-CMP06", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed : 12453413, PubMed : 20581821, PubMed : 27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation : iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed : 27780864). Can also bind siderophores from M.tuberculosis (PubMed : 15642259, PubMed : 21978368).
See full target information LCN2

대체 명칭 보기

HNL, NGAL, LCN2, Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin, p25
style
left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:4453 PubMed39910157

2025

The effect of prednisolone ingestion and acute exercise on lipocalin-2 and its variants in young men: a pilot randomised crossover study.

Applications

Unspecified application

Species

Unspecified reactive species

Carlie Bauer,Rhiannon K Patten,Qiyang Sun,Haoyun Li,Daniels Konja,Mary Woessner,Xuzhu Lin,Andrew Garnham,David L Hare,Peter R Ebeling,Marc Sim,Joshua R Lewis,Yu Wang,Lewan Parker,Itamar Levinger

Journal of inflammation research 14:7035-7051 PubMed34955649

2021

OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fangchen Gong,Ranran Li,Xiangtao Zheng,Weiwei Chen,Yanjun Zheng,Zhitao Yang,Ying Chen,Hongping Qu,Enqiang Mao,Erzhen Chen

Clinical immunology (Orlando, Fla.) 205:65-74 PubMed31212026

2019

Urine macrophages reflect kidney macrophage content during acute tubular interstitial and glomerular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping-Ping Sun,Xu-Jie Zhou,Jian-Qun Su,Chen Wang,Xiao-Juan Yu,Tao Su,Gang Liu,Su-Xia Wang,Jing Nie,Li Yang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com