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AB245714

Human M-CSF ELISA Kit

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(4 제품이 사용된 논문 )

Human M-CSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human M-CSF with a sensitivity of 3.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - Human M-CSF ELISA Kit (AB245714)
  • ELISA

Supplier Data

ELISA - Human M-CSF ELISA Kit (AB245714)
Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)

Interpolated concentrations of native M-CSF in human saliva, urine, and A549 cell supernatant samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 25%, urine 1 : 200, and A549 cell supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 0.9 ng/mL in saliva, 28.1 ng/mL in urine, and 0.89 ng/mL in A549 cell supernatant.

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 27.8 ng/mL with a range of 17.7 - 47.2 ng/mL.

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)

Interpolated concentrations of native M-CSF in human serum, plasma (heparin), and PHA-M stimulated PBMC cell culture supernatant samples. The concentrations of M-CSF were measured in duplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (heparin) 2.5%, and PBMC supernatant 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 11.5 ng/mL in serum, 6.7 ng/mL in plasma (heparin), and 2.4 ng/mL in stimulated PBMC supernatant.

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)

Human M-CSF Standard Curve Comparison. Standard Curve comparison between human M-CSF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)
  • sELISA

Supplier Data

Sandwich ELISA - Human M-CSF ELISA Kit (AB245714)

Example of human M-CSF standard curve in Sample Diluent NS. The M-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Saliva, Urine, Heparin Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3.8 pg/mL

검출 범위

7.8 - 500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human M-CSF ELISA Kit ab245714 is a rapid single-wash 90-min Sandwich ELISA to measure Human M-CSF in cell culture supernatant, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 3.8 pg/mL.

How the assay works

Human M-CSF SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human M-CSF ELISA Kit ab245714 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-M-CSF antibody [EPR21971-6] - BSA and Azide free (Capture) ab259558
- Anti-M-CSF antibody [EPR21971-265] - BSA and Azide free (Detector) ab259558
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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 106 %", "average": "= 95" }, { "sample": "Serum", "range": "114 - 122 %", "average": "= 117" }, { "sample": "Urine", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Saliva", "range": "98 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "107 - 113 %", "average": "= 110" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245714-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB245714-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB245714-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245714-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245714-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245714-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245714-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245714-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human M-CSF Capture Antibody", "number":"AB245714-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human M-CSF Lyophilized recombinant Protein", "number":"AB245714-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human M-CSF Detector Antibody", "number":"AB245714-CMP08", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
Biological function summary

M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

Pathways

The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
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제품 프로토콜

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타겟 정보

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
See full target information CSF1

대체 명칭 보기

Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomaterials research 29:0135 PubMed39911306

2025

A Mechanically Stimulated Co-culture in 3-Dimensional Composite Scaffolds Promotes Osteogenic and Anti-osteoclastogenic Activity and M2 Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia-Ioanna Kontogianni,Konstantinos Loukelis,Amedeo Franco Bonatti,Elisa Batoni,Carmelo De Maria,Giovanni Vozzi,Raasti Naseem,Kenneth Dalgarno,Heungsoo Shin,Chiara Vitale-Brovarone,Maria Chatzinikolaidou

Blood advances 7:5108-5121 PubMed37184294

2023

BET inhibition targets ABC-DLBCL constitutive B-cell receptor signaling through PAX5.

Applications

Unspecified application

Species

Unspecified reactive species

Derek B Oien,Samanta Sharma,Maureen M Hattersley,Michelle DuPont,Steven W Criscione,Laura Prickett,Anne U Goeppert,Lisa Drew,Yi Yao,Jingwen Zhang,Ho Man Chan

Brain, behavior, and immunity 110:348-364 PubMed36940752

2023

Schwann cell insulin-like growth factor receptor type-1 mediates metastatic bone cancer pain in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenzo Landini,Matilde Marini,Daniel Souza Monteiro de Araujo,Antonia Romitelli,Marco Montini,Valentina Albanese,Mustafa Titiz,Alessandro Innocenti,Francesca Bianchini,Pierangelo Geppetti,Romina Nassini,Francesco De Logu

British journal of cancer 127:1142-1152 PubMed35725813

2022

M-CSF as a therapeutic target in BRAF melanoma resistant to BRAF inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

C Barceló,P Sisó,I de la Rosa,C Megino-Luque,R Navaridas,O Maiques,I Urdanibia,N Eritja,X Soria,M Potrony,N Calbet-Llopart,S Puig,X Matías-Guiu,R M Martí,A Macià
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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