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AB277405

Human MEPE ELISA Kit

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Human MEPE ELISA Kit is a Sandwich ELISA for the measurement of Human MEPE in Human in Cell Culture Media, Biofluids samples.
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ELISA - Human MEPE ELISA Kit (AB277405)
  • ELISA

Supplier Data

ELISA - Human MEPE ELISA Kit (AB277405)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8.4 ng/mL

검출 범위

8.192 - 8000 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB270605

Anti-Bone Sialoprotein antibody [EPR23243-30]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23243-30] (AB270605)

  • 0
  • 0
Primary Antibodies

AB154740

Anti-FAM20C antibody

Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (AB154740)

  • 1
  • 1
Assay Kits

AB277706

Human Bone Sialoprotein ELISA Kit

Recombinant|SimpleStep

ELISA - Human Bone Sialoprotein ELISA Kit (AB277706)

  • 0
  • 0
Primary Antibodies

AB272655

Anti-Phospho1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospho1 antibody (AB272655)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human MEPE ELISA Kit (ab277405) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MEPE in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant; Heparin is not recommended for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human MEPE coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MEPE present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MEPE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MEPE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "69 - 95 %", "average": "= 80.13" }, { "sample": "Serum", "range": "69 - 103 %", "average": "= 87.11" }, { "sample": "Plasma", "range": "93 - 105 %", "average": "= 98.58" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277405-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human MEPE coated Microplate", "number":"AB277405-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277405-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277405-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277405-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MEPE standard protein (Lyophilized)", "number":"AB277405-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human MEPE Antibody", "number":"AB277405-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB277405-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MEPE also known as Matrix Extracellular Phosphoglycoprotein is a protein involved in bone metabolism. It has a molecular mass of approximately 56 kDa. MEPE is expressed in osteoblasts and osteocytes cells critical for bone formation and maintenance. This protein also exists in other tissues like kidney and dental pulp where it could possibly have additional roles.
Biological function summary

MEPE contributes to the regulation of bone mineralization and phosphate homeostasis. The protein functions as part of a complex mechanism that modulates bone density responding to signals for bone growth or resorption. Its role involves binding to hydroxyapatite a mineral component of bone which suggests MEPE importantly influences bone matrix structure and strength.

Pathways

MEPE participates in the regulation of the phosphate and vitamin D pathways. It interacts closely with proteins such as PHEX (Phosphate-regulating gene with Homologies to Endopeptidases on the X chromosome) in these pathways. MEPE’s involvement in phosphate regulation affects both renal phosphate handling and intestinal absorption of dietary phosphate hinting at its role in broader systemic calcium and phosphate balance.

MEPE shows connections with disorders like X-linked hypophosphatemic rickets and tumor-induced osteomalacia. These conditions involve abnormal phosphate metabolism and bone degradation. The dysfunction of MEPE along with proteins like PHEX may result in impaired bone mineralization leading to clinical symptoms associated with these conditions such as bone pain and skeletal deformities.
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제품 프로토콜

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타겟 정보

Promotes renal phosphate excretion and inhibits intestinal phosphate absorption (PubMed : 14962809, PubMed : 19005008). Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed : 18162525, PubMed : 19998030, PubMed : 22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity). Promotes dental pulp stem cell proliferation and differentiation (PubMed : 22341070).
See full target information MEPE

대체 명칭 보기

Matrix extracellular phosphoglycoprotein, Osteoblast/osteocyte factor 45, Osteoregulin, OF45, MEPE
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websiteProtocolBooklet
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Product promise

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