AB300333

Human MERTK ELISA Kit - Extracellular

SimpleStep
Recombinant
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Human MERTK ELISA Kit - Extracellular is a Sandwich ELISA for the measurement of Human MERTK - Extracellular in Human in Cell Culture Media, Biofluids samples.

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Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

sELISA

Supplier Data

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Interpolated concentrations of MERTK in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean MERTK concentration was determined to be 74.79 ng/mL with a range of 46.51 –115.95 ng/mL

sELISA

Supplier Data

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Interpolated concentrations of human MERTK in serum, plasma (citrate), plasma (heparin), plasma (EDTA)

Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations. Undiluted samples are 15% serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Example of human MERTK standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Interpolated concentration of native MERTK was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 15% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Example of human MERTK standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Citrate plasma, Heparin Plasma, EDTA Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 90.88 pg/mL

검출 범위

265.625 - 17000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human MERTK SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MERTK protein in human serum, plasma (citrate), plasma (heparin), plasma (EDTA), and cell culture supernatant samples. Quantitate Human MERTK with 90.88 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 97 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "95 - 100 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "93 - 97 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "88 - 96 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "83 - 101 %", "average": "= 94" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

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제품 프로토콜

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타겟 정보

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed : 32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

See full target information MERTK

대체 명칭 보기

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

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websiteProtocolBooklet

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Product promise

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Human MERTK ELISA Kit - Extracellular

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

Sandwich ELISA - Human MERTK ELISA Kit - Extracellular (AB300333)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise