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AB216168

Human Mesothelin ELISA Kit

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(2 제품이 사용된 논문 )

Human Mesothelin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Mesothelin with a sensitivity of 132.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - Human Mesothelin ELISA Kit (AB216168)
  • ELISA

Supplier Data

ELISA - Human Mesothelin ELISA Kit (AB216168)
Sandwich ELISA - Human Mesothelin ELISA Kit (AB216168)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mesothelin ELISA Kit (AB216168)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mesothelin concentration was determined to be 25.79 ng/mL with a range of 6.86 – 60.93 ng/mL.

Sandwich ELISA - Human Mesothelin ELISA Kit (AB216168)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mesothelin ELISA Kit (AB216168)

Interpolated concentrations of native Mesothelin in human serum, plasma, urine and saliva samples.

The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 37.5%, plasma (EDTA) 37.5%, urine 50% and saliva 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mesothelin concentration was determined to be 24.50 ng/mL in serum, 17.17 ng/mL in plasma (heparin), 24.54 ng/mL in plasma (EDTA), 12.37 ng/mL in urine and 277.2 ng/mL in saliva.

Sandwich ELISA - Human Mesothelin ELISA Kit (AB216168)
  • sELISA

Supplier Data

Sandwich ELISA - Human Mesothelin ELISA Kit (AB216168)

Example of human Mesothelin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Saliva, Urine, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 132.5 pg/mL

검출 범위

0.39 - 25 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Mesothelin ELISA Kit ab216168 is a rapid single-wash 90-min Sandwich ELISA to measure Human Mesothelin in cell culture supernatant, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 132.5 pg/mL.

How the assay works

Human Mesothelin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Mesothelin ELISA Kit ab216168 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) ab242775
- Anti-Mesothelin antibody [EPR19025-45] - BSA and Azide free (Detector) ab242775

Mesothelin is a 40 kDa GPI-anchored cell surface protein found on mesothelial cells and overexpressed in several types of cancer. The gene that encodes mesothelin is spliced into two different gene products, mesothelin and megakaryocyte potentiation factor. Mesothelin is involved in cellular adhesion, but is also secreted and normally found in several biological fluids.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "92 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "84 - 95 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "88 - 111 %", "average": "= 98" }, { "sample": "Urine", "range": "85 - 89 %", "average": "= 87" }, { "sample": "Saliva", "range": "100 - 107 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "81 - 87 %", "average": "= 84" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
See full target information MSLN

대체 명칭 보기

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 8:eabm5164 PubMed35984888

2022

Proteomic profiling platforms head to head: Leveraging genetics and clinical traits to compare aptamer- and antibody-based methods.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel H Katz,Jeremy M Robbins,Shuliang Deng,Usman A Tahir,Alexander G Bick,Akhil Pampana,Zhi Yu,Debby Ngo,Mark D Benson,Zsu-Zsu Chen,Daniel E Cruz,Dongxiao Shen,Yan Gao,Claude Bouchard,Mark A Sarzynski,Adolfo Correa,Pradeep Natarajan,James G Wilson,Robert E Gerszten

Molecular therapy oncolytics 16:262-271 PubMed32181327

2020

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhao,Yang Liu,Fuya Zhao,Ye Jin,Jing Feng,Rui Geng,Jiayu Sun,Liqing Kang,Lei Yu,Yunwei Wei
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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