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AB327305

Human MMP12 ELISA Kit, Chemiluminescent

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Human MMP12 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human MMP12 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16.46 - 36000 pg/ml with a sensitivity of 14.97 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human MMP12 ELISA Kit, Chemiluminescent (AB327305)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP12 ELISA Kit, Chemiluminescent (AB327305)

Interpolated concentrations of native MMP12 in human spleen extract based on a 3000 µg/mL extract load.

The concentrations of MMP12 were measured in duplicate and interpolated from the MMP12 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MMP12 concentration was determined to be 7556.82 pg/mL in spleen extract.

Sandwich ELISA - Human MMP12 ELISA Kit, Chemiluminescent (AB327305)
  • sELISA

Lab

Sandwich ELISA - Human MMP12 ELISA Kit, Chemiluminescent (AB327305)

Standard curve comparison between the Chemiluminescent Human MMP12 SimpleStep ELISA and colorimetric Human MMP12 SimpleStep ELISA (ab255715) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP12 ELISA Kit, Chemiluminescent (AB327305)
  • sELISA

Lab

Sandwich ELISA - Human MMP12 ELISA Kit, Chemiluminescent (AB327305)

Dynamic range comparison between the Chemiluminescent Human MMP12 SimpleStep ELISA, colorimetric Human MMP12 SimpleStep ELISA (ab255715), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 14.97 pg/mL

검출 범위

16.46 - 36000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human MMP12 ELISA Kit, Chemiluminescent (ab327305) is a rapid single-wash sandwich 55-min ELISA to measure Human MMP12 in Tissue Extracts with a sensitivity of 14.97 pg/mL.

How the assay works

Human MMP12 ELISA Kit, Chemiluminescent (ab327305) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MMP12 (ab327305) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP-12 antibody [EPR22228-101] - BSA and Aside free (Capture) ab259747
- Anti-MMP-12 antibody [EPR22228-62] - BSA and Aside free (Detector) ab259748

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "81 - 104 %", "average": "= 96" } ]
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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327305-10x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327305-CMP09" }, { "size":"10 x 1 Vial", "name":"Human MMP12 Lyophilized Recombinant Protein", "number":"AB327305-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327305-CMP11" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327305-CMP07" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327305-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327305-CMP05" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327305-CMP10" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327305-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human MMP12 Detector Antibody", "number":"AB327305-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human MMP12 Capture Antibody", "number":"AB327305-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327305-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327305-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327305-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MMP12 Detector Antibody", "number":"AB327305-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327305-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327305-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MMP12 Lyophilized Recombinant Protein", "number":"AB327305-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327305-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327305-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MMP12 Capture Antibody", "number":"AB327305-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327305-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327305-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327305-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
See full target information MMP12

대체 명칭 보기

HME, MMP12, Macrophage metalloelastase, MME, Macrophage elastase, Matrix metalloproteinase-12, ME, hME, MMP-12
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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