AB324503

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular

SimpleStep
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Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular is a Sandwich ELISA for the measurement of Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) - Extracellular in Human, Mouse in Biofluids, Cell Culture Media samples.

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Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Example of human/mouse alpha 1 Sodium Potassium ATPase standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Recombinant alpha 1 Sodium Potassium ATPase was spiked into the following mouse samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and cell culture media (DMEM with 10% FBS). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Specificity and interference testing of human/mouse alpha 1 Sodium Potassium ATPase SimpleStep ELISA kit. 2,500 pg/mL of human (Hu) ATP1A1 and mouse (Ms) ATP1A1 were tested individually for species reactivity. 50 ng/mL each of recombinant mouse (Ms) ATP1A2, ATP1A3, and ATP1A4 were individually tested for specificity, and for interference in combination with 2,500 pg/mL of recombinant mouse ATP1A1. The interpolated values, expressed in % of input (for specificity) or in % of mouse ATP1A1 control (for interference), are plotted (mean +/- SD, n =2). No cross-reactivity or interference was observed with ATP1A2, ATP1A3, and ATP1A4.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Recombinant alpha 1 Sodium Potassium ATPase was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. Undiluted samples are 50% serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Example of human/mouse alpha 1 Sodium Potassium ATPase standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Human, Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 46.942 pg/mL

검출 범위

156.25 - 10000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) with a sensitivity of 46.942pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.2" } ]

Recovery

[ { "sample": "Serum", "range": "79 - 93 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "84 - 110 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "82 - 98 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "90 - 109 %", "average": "= 101" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (PubMed : 17408578).

See full target information Atp1a1

대체 명칭 보기

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise