AB282871

Human MUC2 ELISA Kit

Human MUC2 ELISA 키트

Recombinant
SimpleStep
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Human MUC2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MUC2 with a sensitivity of 0.187 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 이미지

ELISA

Supplier Data

ELISA - Human MUC2 ELISA Kit (AB282871)

sELISA

Supplier Data

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Interpolated concentrations of native MUC2 in human colon and bladder cancer based on 10 μg/mL and 100 µg/mL extract loads, respectively.

The concentrations of MUC2 were measured in duplicate and interpolated from the MUC2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MUC2 concentration was determined to be 27.28 ng/mL in colon tissue extract and 42.10 ng/mL in bladder cancer tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Example of human MUC2 standard curve in Sample Diluent NS.

The MUC2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts, Cell culture media, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.187 ng/mL

검출 범위

0.78 - 50 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human MUC2 ELISA Kit ab282871 is a rapid single-wash 90-min Sandwich ELISA to measure Human MUC2 in cell culture media, serum, tissue extracts. This SimpleStep sensitivity is 0.187 ng/mL.

How the assay works

Human MUC2 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MUC2 ELISA Kit ab282871 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) ab281111
- Anti-MUC2 antibody [EPR23479-91] - BSA and Azide free (Detector) ab281111

MUC2 coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It is thought that MUC2 provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. MUC2 is a major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

Recovery

[ { "sample": "Serum", "range": "83 - 93 %", "average": "= 89" }, { "sample": "Tissue Extracts", "range": "93 - 105 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "96 - 102 %", "average": "= 99" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed : 17058067, PubMed : 19432394, PubMed : 33031746). Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed : 19432394, PubMed : 33031746). MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed : 33031746, PubMed : 36206754). Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed : 36206754). Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites : Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed : 36206754). MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed : 36206754). Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed : 33031746). Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed : 33031746). Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon : a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity). 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).

See full target information MUC2

대체 명칭 보기

SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록and refine your search

EBioMedicine 116:105721 PubMed40344717

2025

Faecal mucoprotein MUC2 is decreased in multiple sclerosis and is associated with mucin degrading bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Luke A Schwerdtfeger,Federico Montini,Tanuja Chitnis,Laura M Cox,Howard L Weiner

Viruses 16: PubMed39066209

2024

Phage-Mediated Digestive Decolonization in a Gut-On-A-Chip Model: A Tale of Gut-Specific Bacterial Prosperity.

Applications

Unspecified application

Species

Unspecified reactive species

Brieuc Van Nieuwenhuyse,Maya Merabishvili,Nathalie Goeders,Kevin Vanneste,Bert Bogaerts,Mathieu de Jode,Joachim Ravau,Jeroen Wagemans,Leïla Belkhir,Dimitri Van der Linden

International journal of molecular sciences 25: PubMed38999957

2024

Effects of High-Mobility Group Box-1 on Mucosal Immunity and Epithelial Differentiation in Colitic Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takamitsu Sasaki,Rina Fujiwara-Tani,Yi Luo,Ruiko Ogata,Rika Sasaki,Ayaka Ikemoto,Yukiko Nishiguchi,Chie Nakashima,Shingo Kishi,Kiyomu Fujii,Hitoshi Ohmori,Naohide Oue,Hiroki Kuniyasu

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Human MUC2 ELISA Kit

Human MUC2 ELISA 키트

ELISA - Human MUC2 ELISA Kit (AB282871)

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

Sandwich ELISA - Human MUC2 ELISA Kit (AB282871)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise