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AB323737

Human MYBPC3 ELISA Kit

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Human MYBPC3 ELISA Kit is a Sandwich ELISA for the measurement of Human MYBPC3 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Human MYBPC3 ELISA Kit (AB323737)
  • sELISA

Supplier Data

Sandwich ELISA - Human MYBPC3 ELISA Kit (AB323737)

Interpolated concentration of native MYBPC3 was measured in duplicate at different sample concentrations. Undiluted samples are 4 μg/mL human heart tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human MYBPC3 ELISA Kit (AB323737)
  • sELISA

Supplier Data

Sandwich ELISA - Human MYBPC3 ELISA Kit (AB323737)

Interpolated concentration of native MYBPC3 was measured in duplicate. Undiluted samples are 4 μg/mL human heart tissue extract, 500 μg/mL human skeletal muscle tissue extract, and 4 μg/mL mouse heart tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW. No signal was detected in human skeletal muscle extract.

Sandwich ELISA - Human MYBPC3 ELISA Kit (AB323737)
  • sELISA

Supplier Data

Sandwich ELISA - Human MYBPC3 ELISA Kit (AB323737)

Example of human MYBPC3 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 11.395 pg/mL

검출 범위

31.25 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Assay Kits

AB314560

Human MYBPC3 Antibody Pair - BSA and Azide free

RabMAb

Sandwich ELISA - Human MYBPC3 Antibody Pair - BSA and Azide free (AB314560)

  • 0
  • 0
Primary Antibodies

AB308619

Anti-MYBPC3 antibody [EPR25673-58]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

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Assay Kits

AB289701

Human Vasopressin-neurophysin 2-copeptin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)

  • 0
  • 0
Proteins & Peptides

AB225662

Recombinant Human MYBPC3 protein (His tag)

SDS-PAGE - Recombinant Human MYBPC3 protein (His tag) (AB225662)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human MYBPC3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MYBPC3 protein in Tissue extracts. Quantitate Human MYBPC3 with 11.395 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "91 - 119 %", "average": "= 106" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
See full target information MYBPC3

대체 명칭 보기

Cardiac MyBP-C, MYBPC3
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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