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AB278115

Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG)

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Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) is a single-wash 90-min Simplestep used to quantify Human Myelin oligodendrocyte glycoprotein (MOG) with a sensitivity of 1.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)
  • sELISA

Supplier Data

Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)

Interpolated concentrations of native Myelin oligodendrocyte glycoprotein in human brain based on a 500 ng/mL extract load

Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)
  • sELISA

Supplier Data

Sandwich ELISA - Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (AB278115)

Example of human Myelin oligodendrocyte glycoprotein standard curve in 1X Cell Extraction Buffer PTR.

The Myelin oligodendrocyte glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Adherent cells, Suspension cells, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.15 pg/mL

검출 범위

6.25 - 400 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human MOG ELISA kit (ab277115) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human MOG protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MOG with 1.15 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Myelin oligodendrocyte glycoprotein (MOG), a 16 kDa protein, mediates homophilic cell-cell adhesion. MOG is a component of the myelin sheath and may be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. Research indicates MOG acts as a receptor for the rubella virus. MOG shares 86%, 86%, 91%, and 98% sequence homology with mouse, rat, cow, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "93 - 97 %", "average": "= 95" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278115-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB278115-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB278115-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278115-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB278115-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278115-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278115-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278115-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278115-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Myelin oligodendrocyte glycoprotein (MOG) Detector Antibody", "number":"AB278115-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Myelin oligodendrocyte glycoprotein (MOG) Capture Antibody", "number":"AB278115-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Myelin oligodendrocyte glycoprotein (MOG) Lyophilized Recombinant Protein", "number":"AB278115-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Myelin oligodendrocyte glycoprotein (MOG) also known as MOG protein or MOG glycoprotein is a lesser-known but important component of the central nervous system myelin. The MOG protein has a molecular mass of approximately 26 to 28 kDa. You will find it expressed on the surface of myelin sheaths and oligodendrocytes. This protein plays a role in the myelination process acting as a potential adhesive molecule or signaling molecule contributing to the stability and integrity of the myelin structure. Oligodendrocyte staining techniques can help visualize the distribution and expression patterns of MOG making it important for research purposes.
Biological function summary

MOG influences the immune response and possibly adhesion between myelin membranes. Although it does not form part of a larger well-defined complex it may interact with other myelin-associated proteins to contribute to myelin construction and maintenance. MOG's involvement in these processes supports myelin sheath formation and function aiding electrical conduction in nerve cells. The protein's exposure on the myelin membrane makes it a possible target for immune attacks hinting at its role in autoimmunity.

Pathways

MOG is involved in immune responses and central nervous system pathways. One significant pathway is the autoimmune pathway where MOG can engage with or be targeted by autoimmune antibodies. It interacts with other proteins like myelin basic protein (MBP) in maintaining myelin structures and influencing immunological functions. This interaction implies that disturbances in these pathways might contribute to numerous neurological disorders.

MOG plays a critical role in conditions such as multiple sclerosis and neuromyelitis optica. In multiple sclerosis MOG is a target of autoantibodies leading to demyelination and loss of neural function. In neuromyelitis optica MOG antibodies may contribute to severe inflammation and damage in optic nerves and spinal cord. The connection between MOG and these diseases highlights associations with other proteins like aquaporin-4 which are similarly targeted in associated autoimmune responses. Understanding these relationships is key for developing diagnostics or therapies involving MOG ELISA methods to detect MOG-specific antibodies in patient samples.
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제품 프로토콜

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타겟 정보

Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.. (Microbial infection) Acts as a receptor for rubella virus.
See full target information MOG

대체 명칭 보기

Myelin-oligodendrocyte glycoprotein, MOG
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websiteProtocolBooklet
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