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AB99997

Human NBL1 ELISA Kit

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Human NBL1 ELISA Kit is a Sandwich ELISA for the measurement of Human NBL1 in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human NBL1 ELISA Kit (AB99997)
  • sELISA

Supplier Data

Sandwich ELISA - Human NBL1 ELISA Kit (AB99997)

Representative Standard Curve using ab99997

Sandwich ELISA - Human NBL1 ELISA Kit (AB99997)
  • sELISA

Supplier Data

Sandwich ELISA - Human NBL1 ELISA Kit (AB99997)

Representative Standard Curve using ab99997

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 35 pg/mL

검출 범위

27.43 - 20000 pg/mL

분석 플랫폼

Microplate

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보완 제품

Proteins & Peptides

AB276217

Recombinant human NBL1 protein (His tag)

Functional Studies - Recombinant human NBL1 protein (His tag) (AB276217)

  • 0
  • 0
Primary Antibodies

AB245033

Anti-NBL1 antibody [EPR18372-106] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-NBL1 antibody [EPR18372-106] - BSA and Azide free (Detector) (AB245033)

  • 0
  • 0
Assay Kits

AB289706

Human Alpha B Crystallin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Alpha B Crystallin ELISA Kit (AB289706)

  • 0
  • 0
Proteins & Peptides

AB211951

Recombinant Human SUGT1 protein

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's NBL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 PARN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "107 - 122 %", "average": "= 111.3" }, { "sample": "Serum", "range": "91 - 109 %", "average": "= 100.1" }, { "sample": "Plasma", "range": "72 - 90 %", "average": "= 81.82" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99997-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99997-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99997-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99997-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99997-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"NBL1 Microplate (12 strips x 8 wells)", "number":"AB99997-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99997-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human NBL1", "number":"AB99997-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human NBL1 Standard (lyophilized)", "number":"AB99997-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB99997-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NBL1 also known as DAN domain family member 1 is a protein with a molecular weight of approximately 20 kDa. It is found in various tissues but shows higher expression levels in the nervous tissue and skeletal muscle. Mechanically NBL1 acts as a modulator in cell signaling pathways by binding to specific growth factors such as BMPs (Bone Morphogenetic Proteins) inhibiting their functions and affecting cell differentiation and growth.
Biological function summary

NBL1 plays an inhibitory role in bone morphogenesis by interacting with BMPs which are part of the TGF-beta superfamily. NBL1 can form complexes with these growth factors thereby regulating processes related to bone and cartilage development. This protein is essential for maintaining cellular processes involved in tissue homeostasis and suppressing unchecked cellular proliferation.

Pathways

NBL1 involves itself in the BMP signaling pathway where it directly impacts the regulation of bone and cartilage development. It is associated with other proteins like Noggin and Chordin both of which also modulate BMP activity highlighting its importance in balancing cell growth and differentiation signals. The involvement of NBL1 in the TGF-beta/BMP pathway is critical for embryonic development and the regulation of body patterning.

NBL1 has been linked to conditions such as osteoarthritis and certain cancer types. Its inhibitory effect on BMPs associates it with osteoarthritis where altered BMP signaling contributes to cartilage degradation. In cancer where BMP activity can be dysregulated NBL1's role in inhibiting BMP pathways places it in connection to tumor suppressors like SMAD proteins emphasizing its potential relevance in tumorigenesis and metastasis.
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제품 프로토콜

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타겟 정보

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
See full target information NBL1

대체 명칭 보기

DAN, DAND1, NBL1, Neuroblastoma suppressor of tumorigenicity 1, DAN domain family member 1, Protein N03, Zinc finger protein DAN
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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