AB267802

Human PAP ELISA Kit (ACPP)

Recombinant
SimpleStep
각 태그의 뜻

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Human PAP ELISA Kit (ACPP) is a single-wash 90-min Simplestep used to quantify Human PAP (ACPP) with a sensitivity of 9.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 416 pg/mL with a range of 94 – 718 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Interpolated concentrations of native PAP in human urine and LNCap cell culture supernatant samples.

The concentrations of PAP were measured in duplicates, interpolated from the PAP standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 10%, and LNCap cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 20,039 pg/mL in neat urine and 5,676 pg/mL in neat LNCap cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Interpolated concentrations of native PAP in human serum and plasma samples.

The concentrations of PAP were measured in duplicates, interpolated from the PAP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAP concentration was determined to be 406 pg/mL in neat serum, 331 pg/mL in neat plasma (citrate), 282 pg/mL in neat plasma (EDTA) and 417 pg/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Example of human PAP standard curve in Sample Diluent NS.

The PAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 9.73 pg/mL

검출 범위

37.5 - 2400 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human PAP ELISA Kit (ACPP) (ab267802) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAP (ACPP) protein in cell culture supernatant, edta plasma, hep plasma, serum, urine, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAP (ACPP) with 9.73 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PAP (Prostatic Acid Phosphatase, EC:3.1.3.2), also known as ACPP is a non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. PAP expression is highest in prostate. PAP is biomarker of prostate cancer as its expression is increased in prostate cancer patients. The isoform 1 is secreted, the isoform 2 is a type I integral membrane protein of plasma membrane and lysosomes. The Isoform 2 function as tumor suppressor through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. The antibodies of this kit are designed to detect both isoforms.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 86 %", "average": "= 99" }, { "sample": "Serum", "range": "84 - 93 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "81 - 98 %", "average": "= 89" }, { "sample": "Urine", "range": "81 - 95 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "89 - 111 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "87 - 106 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "80 - 99 %", "average": "= 90" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed : 10506173, PubMed : 15280042, PubMed : 20498373, PubMed : 9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed : 10506173, PubMed : 15280042).. Isoform 2. Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed : 20498373). In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity).. PAPf39. (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed : 18083097, PubMed : 19451623, PubMed : 19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed : 19451623). Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed : 19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed : 19403677).

See full target information ACP3

대체 명칭 보기

ACPP, ACP3, Prostatic acid phosphatase, PAP, 5'-nucleotidase, Acid phosphatase 3, Ecto-5'-nucleotidase, Protein tyrosine phosphatase ACP3, Thiamine monophosphatase, 5'-NT, TMPase

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in endocrinology 12:830691 PubMed35185790

2022

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Wang,Xiaoyang Fei,Hongyan Zhang,Wenjing Zhou,Zhaojun Cheng,Ying Feng

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Human PAP ELISA Kit (ACPP)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

Sandwich ELISA - Human PAP ELISA Kit (ACPP) (AB267802)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise