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AB209884

Human PCSK9 ELISA Kit

Human PCSK9 ELISA 키트

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(9 제품이 사용된 논문 )

Human PCSK9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PCSK9 with a sensitivity of 68 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
9 이미지
ELISA - Human PCSK9 ELISA Kit (AB209884)
  • ELISA

Supplier Data

ELISA - Human PCSK9 ELISA Kit (AB209884)
Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Supplier Data

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Serum from six individual human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 208 ng/mL with a range of 125 - 317 ng/mL.

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Lab

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Human PCSK9 standard curve comparison data.

Standard curve comparison between human PCSK9 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Supplier Data

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Example of human PCSK9 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Supplier Data

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Example of human PCSK9 standard curve in 1X Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Supplier Data

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract.

The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 50%, HepG2 cell extract 500 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 7.3 ng/mL in HepG2 cell culture supernatant and 15 ng/mL in HepG2 cell extract.

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Unknown

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Interpolated concentrations of native PCSK9 in human serum and plasma samples.

The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 207 ng/mL in serum, 117 ng/mL in plasma (citrate), 166 ng/mL in plasma (EDTA), and 292 ng/mL in plasma (heparin).

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Lab

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Example of human PCSK9 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)
  • sELISA

Lab

Sandwich ELISA - Human PCSK9 ELISA Kit (AB209884)

Interpolated concentration of native PCSK9 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% human serum.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 68 pg/mL

검출 범위

0.5 - 32 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Microplate (12 x 8 well strips)

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보완 제품

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Recombinant|SimpleStep

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AB230932

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Recombinant|SimpleStep

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Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human PCSK9 ELISA Kit ab209884 is a rapid single-wash 90-min Sandwich ELISA to measure Human PCSK9 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 68 pg/mL.

How the assay works

Human PCSK9 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PCSK9 ELISA Kit ab209884 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PCSK9 antibody [EPR18835-113] - BSA and Azide free (Capture) ab242510
- Anti-PCSK9 antibody [EPR18835-18] - BSA and Azide free (Detector) ab242759

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial player in the regulation of plasma cholesterol homeostasis. Degradation of low-density lipid receptor family members (LDLR, VLDLR, APOER, and APOER2) is promoted in intracellular acidic compartments following binding by PCSK9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 109 %", "average": "= 106" }, { "sample": "Serum", "range": "104 - 107 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "93 - 100 %", "average": "= 96" }, { "sample": "Cell culture extracts", "range": "91 - 94 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "107 - 115 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "102 - 106 %", "average": "= 104" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
Biological function summary

PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.

Pathways

PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.

PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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제품 프로토콜

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타겟 정보

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members : low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed : 18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed : 17461796, PubMed : 18197702, PubMed : 18799458, PubMed : 22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed : 18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
See full target information PCSK9

대체 명칭 보기

NARC1, PSEC0052, PCSK9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9
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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록and refine your search

Endokrynologia Polska 76:116-123 PubMed40071808

2025

PCSK9/LOX-1 is associated with T2DM and regulates high glucose-induced lipid metabolism dysfunction in human microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Yao,Hong Liu

Scientific reports 14:15634 PubMed38972879

2024

Assessment of serum proprotein convertase subtilisin/kexin type 9 in pediatric sepsis syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Suzan Omar Mousa,Mohamed Farouk Afifi,Noha Anwar Hassuna,Michael Fekry Yassa,Hend Mohamed Moness

Journal of medical case reports 17:89 PubMed36899379

2023

Injection of an improperly stored proprotein convertase subtilisin/kexin type 9 monoclonal antibody in a patient with secondary dyslipidemia from nephrotic syndrome: a case report.

Applications

Unspecified application

Species

Unspecified reactive species

Tanawan Kongmalai,Nalinee Chuanchaiyakul,Yuttana Srinoulprasert,Nuntakorn Thongtang

Nature biotechnology 41:686-697 PubMed36624149

2023

Improved cytosine base editors generated from TadA variants.

Applications

Unspecified application

Species

Unspecified reactive species

Dieter K Lam,Patricia R Feliciano,Amena Arif,Tanggis Bohnuud,Thomas P Fernandez,Jason M Gehrke,Phil Grayson,Kin D Lee,Manuel A Ortega,Courtney Sawyer,Noah D Schwaegerle,Leila Peraro,Lauren Young,Seung-Joo Lee,Giuseppe Ciaramella,Nicole M Gaudelli

Clinical and translational medicine 12:e711 PubMed35083881

2022

LDLR dysfunction induces LDL accumulation and promotes pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangguang Shi,Yahui Chen,Qingmei Liu,Xueqian Mei,Jing Liu,Yulong Tang,Ruoyu Luo,Dayan Sun,Yanyun Ma,Wenyu Wu,Wenzhen Tu,Yinhuan Zhao,Weihong Xu,Yuehai Ke,Shuai Jiang,Yan Huang,Rui Zhang,Lei Wang,Yuanyuan Chen,Jingjing Xia,Weilin Pu,Honglin Zhu,Xiaoxia Zuo,Yisha Li,Jinhua Xu,Fei Gao,Dong Wei,Jingyu Chen,Wenguang Yin,Qingwen Wang,Huaping Dai,Libing Yang,Gang Guo,Jimin Cui,Nana Song,Hejian Zou,Shimin Zhao,Jörg H W Distler,Li Jin,Jiucun Wang

British journal of pharmacology 178:2461-2481 PubMed33687065

2021

Activation of pregnane X receptor induces atherogenic lipids and PCSK9 by a SREBP2-mediated mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Mikko Karpale,Aki Juhani Käräjämäki,Outi Kummu,Helena Gylling,Tuulia Hyötyläinen,Matej Orešič,Ari Tolonen,Heidi Hautajärvi,Markku J Savolainen,Mika Ala-Korpela,Janne Hukkanen,Jukka Hakkola

Lipids in health and disease 20:21 PubMed33632254

2021

The effect of temperature on the stability of PCSK-9 monoclonal antibody: an experimental study.

Applications

Unspecified application

Species

Unspecified reactive species

Tanawan Kongmalai,Nalinee Chuanchaiyakul,Chattip Sripatumtong,Tunsuda Tansit,Yuttana Srinoulprasert,Nareerak Klinsukon,Nuntakorn Thongtang

The Journal of clinical investigation 130:5223-5234 PubMed32644974

2020

Dengue virus induces PCSK9 expression to alter antiviral responses and disease outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Shuyi Gan,Hwee Cheng Tan,Duyen Huynh Thi Le,Trieu Trung Huynh,Bridget Wills,Nabil G Seidah,Eng Eong Ooi,Sophie Yacoub

Cardiology and therapy 6:281-289 PubMed28623549

2017

Elevated Circulating PCSK9 Concentrations Predict Subclinical Atherosclerotic Changes in Low Risk Obese and Non-Obese Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Štefan Tóth,Ján Fedačko,Tímea Pekárová,Zdenka Hertelyová,Matan Katz,Adil Mughees,Jozef Kuzma,Peter Štefanič,Ivan Kopolovets,Daniel Pella
제품이 사용된 논문 모두 보기
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