AB46068

Human Perforin ELISA Kit (PRF1)

Human Perforin ELISA 키트 (PRF1)

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Human Perforin ELISA Kit (PRF1) is a sandwich ELISA designed to quantify Human Perforin (PRF1) with a sensitivity of 40 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 62.5 - 2000 pg/mL
- Cited in over 10 publications

3 이미지

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

ELISA

Lab

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

Standard curve with background signal subtracted (duplicates; +/- SD).

ELISA

Lab

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

Perforin measured in cell culture supernatants (tested at 1/1-1/100 dilution range; duplicates +/- SD).

sELISA

Unknown

Sandwich ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

Representative standard curve using ab46068

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 40 pg/mL

검출 범위

62.5 - 2000 pg/mL

분석 소요 시간

2h 45m

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Perforin ELISA Kit (PRF1) ab46068 is a sandwich ELISA to measure Human Perforin (PRF1) in cell culture supernatant with a sensitivity of 40 pg/ml.

How the assay works

A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Perforin ELISA Kit (PRF1) ab46068 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 9, "mean": "580 pg/mL", "standardDeviation": "15", "coefficientOfVariability": "3" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 9, "mean": "665 pg/mL", "standardDeviation": "31", "coefficientOfVariability": "5" }, { "reproducibilityType": "Inter", "sample": "Sample C", "replicates": 9, "mean": "1004 pg/mL", "standardDeviation": "45", "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "416 pg/mL", "standardDeviation": "20", "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "674 pg/mL", "standardDeviation": "25", "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Sample C", "replicates": 8, "mean": "984 pg/mL", "standardDeviation": "24", "coefficientOfVariability": "3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 71" } ]

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제품 구성

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46068-2x96Tests", "assetComponentDetails": [ { "size":"4 x 1 Vial", "name":"PRF1 Standard (Lyophilized)", "number":"AB46068-CMP08" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46068-CMP03" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46068-CMP02" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46068-CMP09" }, { "size":"2 x 1 Unit", "name":"PRF1 Microplate (12 x 8 well strips)", "number":"AB46068-CMP07" }, { "size":"2 x 25 mL", "name":"1X Standard Diluent Buffer", "number":"AB46068-CMP10" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46068-CMP05" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46068-CMP01" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46068-CMP04" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti PRF1", "number":"AB46068-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46068-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"PRF1 Standard (Lyophilized)", "number":"AB46068-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46068-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"1X Standard Diluent Buffer", "number":"AB46068-CMP10", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46068-CMP02", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46068-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PRF1 Microplate (12 x 8 well strips)", "number":"AB46068-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46068-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46068-CMP05", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46068-CMP04", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti PRF1", "number":"AB46068-CMP06", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.

Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.

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제품 프로토콜

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타겟 정보

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 20889983, PubMed : 21037563, PubMed : 24558045, PubMed : 9058810, PubMed : 9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed : 9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 20889983, PubMed : 21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 20038786, PubMed : 20225066, PubMed : 24558045, PubMed : 32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed : 24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).

See full target information PRF1

대체 명칭 보기

PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

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제품이 사용된 논문 (16)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Physiological reports 12:e70137 PubMed39632246

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Circ_0089761 accelerates colorectal cancer metastasis and immune escape via miR-27b-3p/PD-L1 axis.

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Qizhong Gao,Xiaowei Cheng,Xiang Gao

Cancer immunology research 12:1691-1702 PubMed39189923

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CAR T Cells Engineered to Secrete IFNκ Induce Tumor Ferroptosis via an IFNAR/STAT1/ACSL4 Axis.

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Journal of extracellular biology 3:e166 PubMed39022723

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Evaluation of resazurin phenoxazine dye as a highly sensitive cell viability potency assay for natural killer cell-derived extracellular vesicle-based cancer biotherapeutics.

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Frederic St-Denis-Bissonnette,Shirley Qiu,Sarah E Cummings,Melanie Kirkby,Yohannes Haile,Sarah Wassmer,Gauri Muradia,Jelica Mehic,Andrew Stalker,Amit Shrestha,Michele Ardolino,Seung-Hwan Lee,Dylan Burger,Lisheng Wang,Jessie R Lavoie

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Define Critical Parameters of Trastuzumab-Mediated ADCC Assays via Assay Optimization Processes, Focusing on the Impact of Cryopreserved Effector Cells on Assay Performance.

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Hanjing Peng,Yukinori Endo,Wen Jin Wu

Frontiers in immunology 15:1376096 PubMed38863707

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Structural and functional characterization of IgG- and non-IgG-based T-cell-engaging bispecific antibodies.

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Nishant Mohan,Safiat Ayinde,Hanjing Peng,Shraboni Dutta,Yi Shen,Vincent M Falkowski,Thomas G Biel,Tongzhong Ju,Wen Jin Wu

Science advances 9:eadj9964 PubMed38134285

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Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.

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Ruoyu Zhong,Matthew Sullivan,Neil Upreti,Roy Chen,Agustin De Ganzó,Kaichun Yang,Shujie Yang,Ke Jin,Ye He,Ke Li,Jianping Xia,Zhiteng Ma,Luke P Lee,Tania Konry,Tony Jun Huang

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 56:e12997 PubMed38126537

2023

The level of Tim-3+CD8+ T cells can serve as a potential marker for evaluating the severity of acute graft-versus-host disease after haplo-PBSCT.

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Nannan Pang,Mingkai Yu,Jianli Xu,Hailong Yuan,Gang Chen,Dong Wang,Chunxia Han,Weiguo Wang,Jianbing Ding,Ming Jiang

Nature communications 14:7461 PubMed37985656

2023

Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females.

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Christophe M Capelle,Séverine Ciré,Fanny Hedin,Maxime Hansen,Lukas Pavelka,Kamil Grzyb,Dimitrios Kyriakis,Oliver Hunewald,Maria Konstantinou,Dominique Revets,Vera Tslaf,Tainá M Marques,Clarissa P C Gomes,Alexandre Baron,Olivia Domingues,Mario Gomez,Ni Zeng,Fay Betsou,Patrick May,Alexander Skupin,Antonio Cosma,Rudi Balling,Rejko Krüger,Markus Ollert,Feng Q Hefeng

Journal for immunotherapy of cancer 9: PubMed34244307

2021

Combinatorial immunotherapy of N-803 (IL-15 superagonist) and dinutuximab with ex vivo expanded natural killer cells significantly enhances in vitro cytotoxicity against GD2 pediatric solid tumors and in vivo survival of xenografted immunodeficient NSG mice.

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Yaya Chu,Gaurav Nayyar,Susiyan Jiang,Jeremy M Rosenblum,Patrick Soon-Shiong,Jeffrey T Safrit,Dean A Lee,Mitchell S Cairo

Cancer immunology research 8:1273-1286 PubMed32847938

2020

An IL6-Adenosine Positive Feedback Loop between CD73 γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer.

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Guoming Hu,Pu Cheng,Jun Pan,Shimin Wang,Qiannan Ding,Zhou Jiang,Lu Cheng,Xuan Shao,Liming Huang,Jian Huang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Human Perforin ELISA Kit (PRF1)

Human Perforin ELISA 키트 (PRF1)

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

Sandwich ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (16)

Product promise