AB289907

Human PLTP ELISA Kit

SimpleStep
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Human PLTP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PLTP with a sensitivity of 46.254 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 이미지

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

sELISA

Supplier Data

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

Representative image of Human PLTP ELISA Kit (ab289907)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human PLTP Capture Antibody

- 10X Human PLTP Detector Antibody

- Human PLTP Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

Interpolated concentrations of human PLTP in U87 extract, JAR extract, and PC-3 extract.

Interpolated concentration of native PLTP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : U87 extract 31.25 μg/mL, JAR extract 62.5 μg/mL, PC-3 extract 62.5 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Unknown

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

Example of human PLTP standard curve in 1X Cell Extraction Buffer PTR.

Example of human PLTP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

>= 46.254 pg/mL

검출 범위

125 - 8000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Human PLTP ELISA Kit (ab289907) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PLTP in cell lysate samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PLTP with 46.254 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.4" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "83 - 112 %", "average": "= 102" } ]

style

left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289907-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB289907-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB289907-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289907-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289907-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289907-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289907-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB289907-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289907-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289907-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PLTP Capture Antibody", "number":"AB289907-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PLTP Lyophilized Recombinant Protein", "number":"AB289907-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PLTP Detector Antibody", "number":"AB289907-CMP08", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PLTP also known as Phospholipid Transfer Protein is an important component in human lipid metabolism. This protein functions by transferring phospholipids between different types of lipoproteins in plasma. PLTP possesses a molecular mass of about 56 kDa. Its expression occurs mainly in the liver and adipose tissue with additional presence in the brain and lungs. These locales of expression facilitate its key roles in lipid processing and lipoprotein metabolism.

Biological function summary

PLTP plays a significant role in remodeling high-density lipoproteins (HDL) and regulating lipoprotein particles. PLTP exists as a part of a larger complex involved in these processes interacting closely with other proteins in the lipoprotein maturation and transformation. This activity of PLTP significantly affects the levels and sizes of HDL particles in circulation thereby influencing cholesterol homeostasis within the body.

Pathways

PLTP acts within the reverse cholesterol transport pathway important for the removal of excess cholesterol from peripheral tissues. The protein works alongside Apolipoprotein A-I (ApoA-I) and Lecithin-Cholesterol Acyltransferase (LCAT) to promote cholesterol efflux and HDL maturation. Another key pathway involving PLTP is the lipid metabolism pathway where it influences lipid levels and impacts the function of other proteins such as Apolipoprotein E (ApoE).

PLTP has associations with atherosclerosis and metabolic syndrome. Variations in PLTP activity or expression can lead to altered HDL levels increasing the risk for heart disease and related cardiovascular conditions. In atherosclerosis PLTP's relationship with ApoA-I becomes especially relevant as this interaction influences lipid transport dynamics and plaque formation. Additionally in metabolic syndrome abnormal PLTP function may contribute to dyslipidemia a common factor in metabolic disorders.

style

left-column

제품 프로토콜

style

left-column

타겟 정보

Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves (PubMed : 11013307, PubMed : 19321130, PubMed : 21515415, PubMed : 29883800, PubMed : 7654777, PubMed : 9132017). Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine (PubMed : 9132017). Plays an important role in HDL remodeling which involves modulating the size and composition of HDL (PubMed : 29883800). Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion (PubMed : 21736953). Two distinct forms of PLTP exist in plasma : an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability (PubMed : 11013307).

See full target information PLTP

대체 명칭 보기

Phospholipid transfer protein, Lipid transfer protein II, PLTP

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PLTP ELISA Kit

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

Sandwich ELISA - Human PLTP ELISA Kit (AB289907)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise