JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327071

Human PR3 ELISA Kit, Chemiluminescent

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human PR3 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human PR3 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.14 - 300 ng/ml with a sensitivity of 56 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
3 이미지
Sandwich ELISA - Human PR3 ELISA Kit, Chemiluminescent (AB327071)
  • sELISA

Supplier Data

Sandwich ELISA - Human PR3 ELISA Kit, Chemiluminescent (AB327071)

Interpolated concentrations of native PR3 in Human cell extracts and cell culture supernatant samples.

The concentrations of PR3 were measured in duplicates interpolated from the PR3 standard curves and corrected for sample dilution. Undiluted samples are as follows : THP-1 cell extract (100 ug/ml); HL-60 (50 ug/ml); untreated THP-1 cell supernatant (100 %) and untreated Human PBMC supernatant (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PR3 concentration was determined to be 24.62 ng/mL in THP-1 cell extract 72.65 ng/mL in HL-60 cell extract and 6.16 ng/mL in THP-1 supernatant and 146.21 ng/ml in PBMC supernatant.

Sandwich ELISA - Human PR3 ELISA Kit, Chemiluminescent (AB327071)
  • sELISA

Lab

Sandwich ELISA - Human PR3 ELISA Kit, Chemiluminescent (AB327071)

Standard curve comparison between the Chemiluminescent Human PR3 SimpleStep ELISA and colorimetric Human PR3 SimpleStep ELISA (colorimetric ab226902) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PR3 ELISA Kit, Chemiluminescent (AB327071)
  • sELISA

Lab

Sandwich ELISA - Human PR3 ELISA Kit, Chemiluminescent (AB327071)

Dynamic range comparison between the Chemiluminescent Human PR3 SimpleStep ELISA and colorimetric Human PR3 SimpleStep ELISA (colorimetric ab226902), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Cell culture supernatant, Cell culture extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 56 pg/mL

검출 범위

0.14 - 300 ng/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human PR3 ELISA Kit, Chemiluminescent (ab327071) is a rapid single-wash sandwich 55-min ELISA to measure Human PR3 in Cell culture supernatant, Cell culture extracts with a sensitivity of 56 pg/mL.

How the assay works

Human PR3 ELISA Kit, Chemiluminescent (ab327071) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PR3 (ab327071) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PR3 antibody [EPR20649-107] - BSA and Azide free (Capture) ab244683
- Anti-PR3 antibody [EPR20649-109] - BSA and Azide free (Detector) ab244949

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 117 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "87 - 93 %", "average": "= 89" } ]
style
left-column

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327071-10x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327071-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327071-CMP08" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327071-CMP05" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327071-CMP10" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327071-CMP07" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327071-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327071-CMP12" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327071-CMP11" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327071-CMP04" }, { "size":"10 x 600 µL", "name":"10X Human PR3 Capture Antibody", "number":"AB327071-CMP01" }, { "size":"10 x 1 Vial", "name":"Human PR3 Lyophilized Recombinant Protein", "number":"AB327071-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human PR3 Detector Antibody", "number":"AB327071-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327071-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327071-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327071-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PR3 Lyophilized Recombinant Protein", "number":"AB327071-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PR3 Capture Antibody", "number":"AB327071-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327071-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327071-CMP12", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327071-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327071-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB327071-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB327071-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PR3 Detector Antibody", "number":"AB327071-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327071-CMP04", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed : 2033050, PubMed : 28240246, PubMed : 3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed : 23202369). Plays a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed : 22266279). Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation (PubMed : 36302784).
See full target information PRTN3

대체 명칭 보기

MBN, PRTN3, Myeloblastin, AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, P29, Wegener autoantigen, PR-3, PR3, NP-4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com