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AB325999

Human Prekallikrein 1B ELISA Kit, Chemiluminescent

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Human Prekallikrein 1B ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Prekallikrein 1B with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 85.73 - 62500 pg/ml with a sensitivity of 22.12 pg/mL.
- Patent pending
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Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)
  • sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)
  • sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Standard curve comparison between the Chemiluminescent Human Prekallikrein 1B SimpleStep ELISA and colorimetric Human Prekallikrein 1B SimpleStep ELISA (ab202405) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)
  • sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prekallikrein 1B concentration was determined to be 112.6 µg/mL with a range of 80.8 – 206.4 µg/mL in male donors.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)
  • sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Titration of human serum and plasma within the working range of the assay. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)
  • sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Dynamic range comparison between the Chemiluminescent Human Prekallikrein 1B SimpleStep ELISA and colorimetric Human Prekallikrein 1B SimpleStep ELISA (ab202405). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Milk, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 22.12 pg/mL

검출 범위

85.73 - 62500 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Prekallikrein 1B ELISA Kit, Chemiluminescent (ab325999) is a rapid single-wash sandwich 55-min ELISA to measure Human Prekallikrein 1B in Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Milk, Cell culture supernatant with a sensitivity of 22.12 pg/mL.

How the assay works

Human Prekallikrein 1B ELISA Kit, Chemiluminescent (ab325999) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Prekallikrein 1B (ab325999) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 101 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "82 - 91 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "88 - 107 %", "average": "= 101" }, { "sample": "Milk", "range": "87 - 97 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "87 - 95 %", "average": "= 92" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325999-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325999-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Prekallikrein 1B Capture Antibody", "number":"AB325999-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325999-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325999-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325999-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Prekallikrein 1B Lyophilized Recombinant Protein", "number":"AB325999-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Prekallikrein 1B Detector Antibody", "number":"AB325999-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325999-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325999-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325999-CMP07", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
See full target information KLKB1

대체 명칭 보기

KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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