AB303757

Human Pro-MCH ELISA Kit

Recombinant
SimpleStep
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Human Pro-MCH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Pro-MCH with a sensitivity of 100.411 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human Pro-MCH ELISA Kit (AB303757)

sELISA

Supplier Data

Sandwich ELISA - Human Pro-MCH ELISA Kit (AB303757)

Interpolated concentrations of human Pro-MCH in tissue extract

Interpolated concentration of native Pro-MCH was measured in duplicate at different sample concentrations. Undiluted sample are 200 µg/mL human brain hippocampus extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Pro-MCH ELISA Kit (AB303757)

Example of human Pro-MCH standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 100.411 pg/mL

검출 범위

187.5 - 12000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Pro-MCH SimpleStep ELISA® kit (ab303757) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Pro-MCH protein in tissue extracts. Quantitate Human Pro-MCH with 100.411 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

Recovery

[ { "sample": "Tissue Extracts", "range": "106 - 110 %", "average": "= 108" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303757-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB303757-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303757-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303757-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303757-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB303757-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303757-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303757-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB303757-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB303757-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Pro-MCH Capture Antibody", "number":"AB303757-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Pro-MCH Lyophilized Recombinant Protein", "number":"AB303757-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Pro-MCH Detector Antibody", "number":"AB303757-CMP03", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pro-MCH also known as pro-melanin-concentrating hormone is a precursor polypeptide that undergoes post-translational processing to produce mature melanin-concentrating hormone (MCH). The precursor weighs approximately 18 kDa before processing. Pro-MCH is expressed mainly in the hypothalamus but can also be found in other brain regions involved in regulating appetite and energy homeostasis.

Biological function summary

The pro-MCH precursor undergoes cleavage to generate MCH which plays a significant role in energy balance and feeding behavior. MCH functions are often linked to neuronal populations in the hypothalamus that regulate hunger signals and it is involved in forming neuronal circuits that influence behavior related to food intake. Although pro-MCH itself is not part of a larger complex the derived MCH interacts with its receptors to transmit signals.

Pathways

Melanin-concentrating hormone interacts with energy homeostasis and reward circuits in the brain. It is engaged in the melanocortin pathway which balances energy intake and expenditure. MCH modulates signaling by interacting with receptors such as MCHR1 and MCHR2 influencing food-seeking behavior and energy regulation processes. Additionally its regulation aligns with orexigenic (appetite-increasing) peptides such as neuropeptide Y (NPY) coordinating energy-related responses.

Aberrant MCH signaling relates to metabolic disorders such as obesity and altered response to stress. Individuals with irregular MCH signaling may exhibit increased appetite and weight gain contributing to metabolic syndrome. The interaction with MCHR1 is significant here as alterations in this receptor are associated with such metabolic changes. Furthermore pro-MCH and its derivative peptides have also been linked to mood disorders with disrupted MCH pathways influencing depression-related behaviors through interactions with proteins like corticotropin-releasing hormone (CRH).

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제품 프로토콜

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타겟 정보

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.

See full target information PMCH

대체 명칭 보기

MCH, PMCH, Pro-MCH

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websiteProtocolBooklet

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Product promise

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Human Pro-MCH ELISA Kit

Sandwich ELISA - Human Pro-MCH ELISA Kit (AB303757)

Sandwich ELISA - Human Pro-MCH ELISA Kit (AB303757)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Primary Antibodies

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise