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AB226901

Human Prolactin/PRL ELISA Kit

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(5 제품이 사용된 논문 )

Human Prolactin/PRL ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prolactin/PRL with a sensitivity of 17.64 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
6 이미지
ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
  • ELISA

Supplier Data

ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
  • sELISA

Unknown

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)

Human Prolactin Competitor standard curve comparison

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)

Interpolated concentrations of native Prolactin in human pregnant serum and milk.

The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 42,647 pg/mL in pregnant serum and 1,214 pg/mL in milk.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)

Serum from ten individual healthy human female donors and ten individual healthy male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration in females was determined to be 2,778 pg/mL with a range of 294 – 18,132 pg/mL. The mean Prolactin concentration in males was determined to be 1,733 pg/mL with a range of 179 – 7,626 pg/mL.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)

Interpolated concentrations of native Prolactin in human serum and plasma and of spiked Prolactin in cell culture media samples.

The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 1,066 pg/mL in serum, 480 pg/mL in plasma (citrate), 801 pg/mL in plasma (heparin), and 711 pg/mL in plasma (EDTA).

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit (AB226901)

Example of human Prolactin standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 17.64 pg/mL

검출 범위

39.06 - 2500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Prolactin/PRL ELISA Kit ab226901 is a rapid single-wash 90-min Sandwich ELISA to measure Human Prolactin/PRL in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, serum. This SimpleStep sensitivity is 17.64 pg/mL.

How the assay works

Human Prolactin/PRL SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Prolactin/PRL ELISA Kit ab226901 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Prolactin/PRL antibody [EPR20580-116] - BSA and Azide free (Capture) ab244948
- Anti-Prolactin/PRL antibody [EPR20580-114] - BSA and Azide free (Detector) ab244948

Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14" } ]
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Recovery

[ { "sample": "Milk", "range": "100 - 109 %", "average": "= 106" }, { "sample": "Serum", "range": "86 - 91 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "76 - 82 %", "average": "= 79" }, { "sample": "Heparin Plasma", "range": "88 - 91 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "70 - 82 %", "average": "= 76" }, { "sample": "Cell culture media", "range": "108 - 118 %", "average": "= 114" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.
Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.
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제품 프로토콜

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타겟 정보

See full target information Prolactin/PRL

대체 명칭 보기

Decidual prolactin, GHA1, Growth hormone A1, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, Prolactin, Prolactin precursor
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International breastfeeding journal 20:14 PubMed40051012

2025

Effectiveness of a new breastfeeding counselling intervention on breastfeeding prevalence, infant growth velocity and postpartum weight loss in overweight women: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Aldana-Parra,Gilma Olaya Vega,Mary Fewtrell

Communications biology 6:568 PubMed37244968

2023

JAZF1 safeguards human endometrial stromal cells survival and decidualization by repressing the transcription of G0S2.

Applications

Unspecified application

Species

Unspecified reactive species

Yingyu Liang,Siying Lai,Lijun Huang,Yulian Li,Shanshan Zeng,Shuang Zhang,Jingsi Chen,Wenbo Deng,Yu Liu,Jingying Liang,Pei Xu,Mingxing Liu,Zhongtang Xiong,Dunjin Chen,Zhaowei Tu,Lili Du

Comprehensive psychoneuroendocrinology 6:100049 PubMed35757357

2021

Plasma prolactin is higher in major depressive disorder and females, and associated with anxiety, hostility, somatization, psychotic symptoms and heart rate.

Applications

Unspecified application

Species

Unspecified reactive species

Asmahan Elgellaie,Theresa Larkin,Jacqueline Kaelle,Jessica Mills,Susan Thomas

International breastfeeding journal 15:2 PubMed31921328

2020

Effectiveness of a new approach for exclusive breastfeeding counselling on breastfeeding prevalence, infant growth velocity and postpartum weight loss in overweight or obese women: protocol for a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Aldana-Parra,Gilma Olaya,Mary Fewtrell

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 109:1578-1585 PubMed30551411

2018

Metformin inhibits estradiol and progesterone-induced decidualization of endometrial stromal cells by regulating expression of progesterone receptor, cytokines and matrix metalloproteinases.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Xiong,Juxia Xiao,Yinyang Bai,Yun Zhang,Qiuping Li,Xu Lishuang
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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