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AB283989

Human PSPH ELISA Kit

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Human PSPH ELISA Kit is a Sandwich ELISA for the measurement of Human PSPH in Human in Cell/Tissue Extracts samples.

대체 명칭 보기

Phosphoserine phosphatase, PSP, PSPase, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSPH

1 이미지
Sandwich ELISA - Human PSPH ELISA Kit (AB283989)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSPH ELISA Kit (AB283989)

Example of Human PSPH standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.2 ng/mL

검출 범위

0.313 - 10 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Proteins & Peptides

AB48755

Recombinant Human PSPH protein (Tag Free)

  • 0
  • 0
Primary Antibodies

AB9332

Anti-Phosphoserine antibody

Western blot - Anti-Phosphoserine antibody (AB9332)

  • 3
  • 9
Primary Antibodies

AB307321

Anti-PSPH antibody [EPR26995-3] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-PSPH antibody [EPR26995-3] - BSA and Azide free (Detector) (AB307321)

  • 0
  • 0
Primary Antibodies

AB117253

Anti-Phospho - (Ser/Thr) antibody

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

The Human PSPH ELISA Kit (ab283989) is designed for detection of PSPH in human cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PSPH in approximately 5 hours. A polyclonal antibody specific for human PSPH has been pre-coated onto a 96-well microplate with removable strips. PSPH in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human PSPH, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoserine phosphatase (PSPH) also known as O-phosphoserine phosphohydrolase plays an essential role in serine biosynthesis by catalyzing the conversion of O-phospho-L-serine to L-serine. PSPH is a protein with a molecular weight of approximately 25 kDa. It is expressed across a wide range of tissues with notable expression in the liver kidney and brain. The enzyme activity of PSPH ensures the availability of serine a non-essential amino acid involved in multiple biochemical processes.
Biological function summary

Phosphoserine phosphatase contributes significantly to cellular metabolism and growth by its involvement in the serine biosynthesis pathway. This pathway is important for providing serine which acts as a precursor for proteins nucleotides and other biologically important molecules. PSPH does not function as part of a larger complex; however its enzymatic action is critical for maintaining levels of serine when dietary intake is low. In addition to its role in metabolism PSPH influences cell proliferation and differentiation through providing the necessary serine reserves.

Pathways

Phosphoserine phosphatase integrates into the serine biosynthesis pathway and shows a connection to the glycolytic pathway. The enzyme catalyzes one of the final steps in the phosphorylated serine synthesis process that branches from glycolysis. This pathway features interactions with enzymes like 3-phosphoglycerate dehydrogenase (PHGDH) and phosphoserine aminotransferase 1 (PSAT1) which work sequentially to produce 3-phosphoserine from 3-phosphoglycerate. PSPH follows these enzymes completing the conversion to serine and connects the serine biosynthesis cycle to broader metabolic activities.

Altered expression or function of phosphoserine phosphatase associates with conditions such as cancer and neurodegenerative diseases. Within oncogenesis elevated activity of PSPH can supply cancer cells with serine needed for rapid proliferation and this enzyme interplays with other metabolic proteins like PHGDH in cancer metabolism. In neurodegenerative disorders deficient PSPH activity disrupts serine availability leading to harmful effects on the nervous system. Understanding PSPH's role in these conditions can provide insights into therapeutic strategies targeting serine metabolism.
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제품 프로토콜

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타겟 정보

Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine (PubMed : 12213811, PubMed : 14673469, PubMed : 15291819, PubMed : 25080166, PubMed : 9222972). L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator (PubMed : 14673469). May also act on O-phospho-D-serine (Probable).
See full target information Phosphoserine phosphatase
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websiteProtocolBooklet
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Product promise

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