AB317542

Human S100 alpha 6/PRA ELISA Kit

SimpleStep
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Human S100 alpha 6/PRA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human S100 alpha 6/PRA with a sensitivity of 0.789 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA

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Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Interpolated concentrations of human S100 alpha 6/PRA in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin)

Interpolated concentration of native S100 alpha 6/PRA was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 human serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Interpolated concentrations of human S100 alpha 6/PRA in human serum, Rhesus macaque serum, and Cynomolgus monkey serum

Interpolated concentration of native S100 alpha 6/PRA was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Interpolated concentrations of human S100 alpha 6/PRA in human urine and A549 supernatant

Interpolated concentration of native S100 alpha 6/PRA was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 1 : 40 and A549 supernatant 1 : 50. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Interpolated concentrations of human S100 alpha 6/PRA in human serum in 96-well vs. 384-well plates

Interpolated concentration of native S100 alpha 6/PRA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 200 human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Example of human S100 alpha 6/PRA standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Example of human S100 alpha 6/PRA standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Urine, Cell culture supernatant, Citrate plasma, Heparin Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.789 pg/mL

검출 범위

2.734 - 175 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human S100 alpha 6/PRA SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100 alpha 6/PRA protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Cell culture supernatant. Quantitate Human S100 alpha 6/PRA with 0.789 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Serum", "range": "93 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "84 - 100 %", "average": "= 92" }, { "sample": "Urine", "range": "102 - 112 %", "average": "= 108" }, { "sample": "Cell culture supernatant", "range": "99 - 111 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "95 - 108 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "91 - 103 %", "average": "= 96" } ]

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317542-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB317542-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317542-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317542-CMP07" }, { "size":"20 x 1 Vial", "name":"Human S100 alpha 6/PRA Lyophilized Recombinant Protein", "number":"AB317542-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human S100 alpha 6/PRA Capture Antibody", "number":"AB317542-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent BAR", "number":"AB317542-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317542-CMP05" }, { "size":"10 x 600 µL", "name":"10X Human S100 alpha 6/PRA Detector Antibody", "number":"AB317542-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317542-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317542-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB317542-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317542-CMP08" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317542-CMP07" }, { "size":"1 x 6 mL", "name":"Antibody Diluent BAR", "number":"AB317542-CMP04" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317542-CMP05" }, { "size":"2 x 1 Vial", "name":"Human S100 alpha 6/PRA Lyophilized Recombinant Protein", "number":"AB317542-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human S100 alpha 6/PRA Capture Antibody", "number":"AB317542-CMP01" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317542-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human S100 alpha 6/PRA Detector Antibody", "number":"AB317542-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317542-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB317542-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317542-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317542-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317542-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent BAR", "number":"AB317542-CMP04", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317542-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human S100 alpha 6/PRA Capture Antibody", "number":"AB317542-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human S100 alpha 6/PRA Lyophilized Recombinant Protein", "number":"AB317542-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human S100 alpha 6/PRA Detector Antibody", "number":"AB317542-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317542-CMP09", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha 6 also known as PRA is a member of the S100 protein family characterized by its small molecular weight of approximately 10 kDa. This protein expresses in various tissues including the heart skeletal muscle kidney and brain. The S100 family proteins commonly contain two EF-hand calcium-binding motifs which play key roles in intracellular and extracellular regulatory activities. S100 alpha 6 participates in calcium-dependent signal transduction processes that affect a range of cellular functions.

Biological function summary

S100 alpha 6 plays a significant role in intracellular calcium signaling and cytoskeletal dynamics. This protein often interacts as a part of protein complexes with various other proteins including annexin and tropomyosin contributing to vital cellular functions like contraction motility and cell cycle regulation. Its expression levels and spatial distribution influence cellular proliferation and differentiation making it an important component in several cell types.

Pathways

The protein S100 alpha 6 participates in essential pathways such as the MAPK and calcium signaling pathways. In the MAPK pathway S100 alpha 6 interacts with proteins like ERK1/2 influencing cell growth and apoptosis signaling cascades. In calcium signaling it helps regulate the release and uptake of calcium ions vital for maintaining cellular homeostasis and function. Through these pathways S100 alpha 6 is interconnected with other proteins like calmodulin and calcyclin which share similar physiological roles.

Increased or decreased expression of S100 alpha 6 relates to conditions such as cancer and cardiomyopathy. In cancer S100 alpha 6 often associates with dysregulated cell growth and can influence tumor progression through its interactions with proteins such as p53 and MDM2. In cardiomyopathy it affects calcium-handling properties in heart muscle cells linking it to cardiac dysfunction. These associations highlight its impact on disease development and progression making it a target for research in therapeutic strategies.

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제품 프로토콜

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타겟 정보

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.. (Microbial infection) Facilitates attachment and invasion of Toxoplasma gondii parasites.

See full target information S100A6

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human S100 alpha 6/PRA ELISA Kit

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

Sandwich ELISA - Human S100 alpha 6/PRA ELISA Kit (AB317542)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise