Human S100A12/CGRP ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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Human S100A12/CGRP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human S100A12/CGRP with a sensitivity of 1.89 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
대체 명칭 보기
Protein S100-A12, CGRP, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, Extracellular newly identified RAGE-binding protein, Migration inhibitory factor-related protein 6, Neutrophil S100 protein, S100 calcium-binding protein A12, CAAF1, CAGC, EN-RAGE, MRP-6, p6, S100A12
- ELISA
Supplier Data
ELISA - Human S100A12/CGRP ELISA Kit (AB282299)
- sELISA
Supplier Data
Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A12/CGRP concentration was determined to be 2,155 pg/mL with a range of 1,217 – 39,456 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)
Interpolated concentrations of native S100A12/CGRP in human serum and plasma samples.
The concentrations of S100A12/CGRP were measured in duplicates, interpolated from the S100A12/CGRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20, plasma (citrate) 1 : 8, plasma (EDTA) 1 : 8, and plasma (heparin) 1 : 16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A12/CGRP concentration was determined to be 6,428 pg/mL in serum, 5,285 pg/mL in plasma (citrate), 3,163 pg/mL in plasma (EDTA), and 2,034 pg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)
Example of human S100A12/CGRP standard curve in Sample Diluent NS.
The S100A12/CGRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
보완 제품
Reactivity 정보
제품 세부 정보
Human S100A12/CGRP ELISA Kit ab282299 is a rapid single-wash 90-min Sandwich ELISA to measure Human S100A12/CGRP in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.89 pg/mL.
How the assay works
Human S100A12/CGRP SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human S100A12/CGRP ELISA Kit ab282299 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) ab281062
- Anti-S100A12/CGRP antibody [EPR23677-102] - BSA and Azide free (Detector) ab281062
S100A12, also known as CCRP and calgranulin C, is encoded by the S100A12 gene and a member of the S100 family. Found secreted by neutrophils, monocytes, and keratinocytes in which the protein plays significant roles as antiparasitic, anti-infectious, and antibacterial. Moreover, oligomerization and target interactions by S100A12 is regulated by both zinc and calcium ions resulting in cell signaling and changes in cellular microenvironment.
Precision
Recovery
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보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
S100A12 is important in the inflammatory response and immune system regulation. It acts as a pro-inflammatory mediator attracting leukocytes to sites of inflammation. Furthermore it can bind to receptors on cell surfaces like RAGE (Receptor for Advanced Glycation End Products) which induces signaling cascades that amplify immune responses. S100A12 does not function alone but often forms homodimers or heterodimers with other S100 proteins enhancing its biological activities.
Pathways
S100A12 participates in pathways like the inflammatory response pathway and the RAGE signaling pathway. By interacting with RAGE and possibly other receptors it impacts cellular responses associated with stress injury and microbial infection. This interaction connects S100A12 to other proteins in the same pathways such as NF-kB an important transcription factor involved in inflammation and immune responses.
타겟 정보
제품이 사용된 논문 (2)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Annals of medicine 57:2451194 PubMed39823189
2025
Applications
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Species
Unspecified reactive species
International journal of molecular sciences 23: PubMed35886858
2022
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Unspecified reactive species
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