JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309446

Human SAHH ELISA Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human SAHH ELISA Kit is a Sandwich ELISA for the measurement of Human SAHH in Human in Biofluids, Cell Culture Media samples.

대체 명칭 보기

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase

1 이미지
Sandwich ELISA - Human SAHH ELISA Kit (AB309446)
  • sELISA

Supplier Data

Sandwich ELISA - Human SAHH ELISA Kit (AB309446)

Example of standard curve using ab309446. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.61 ng/mL

검출 범위

0.61 - 150 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Proteins & Peptides

AB74521

Recombinant Human CHOP protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CHOP protein (His tag N-Terminus) (AB74521)

  • 0
  • 0
Assay Kits

AB253597

Human CHOP Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human CHOP Antibody Pair - BSA and Azide free (AB253597)

  • 0
  • 0
Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

  • 4
  • 3
Assay Kits

AB228559

Homocysteine Assay Kit (Fluorometric)

ELISA - Homocysteine Assay Kit (Fluorometric) (AB228559)

  • 1
  • 1
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human SAHH ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAHH in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human SAHH coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "119 - 127 %", "average": "= 123.8" }, { "sample": "Plasma", "range": "77 - 119 %", "average": "= 107" }, { "sample": "Cell culture supernatant", "range": "97 - 117 %", "average": "= 107.4" }, { "sample": "Cell culture media", "range": "97 - 117 %", "average": "= 107.4" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309446-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309446-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SAHH Protein Standard", "number":"AB309446-CMP17", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309446-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SAHH Antibody-coated ELISA Plate", "number":"AB309446-CMP15", "productcode":"AB309446" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB309446-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309446-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309446-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309446-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SAHH Detection Antibody", "number":"AB309446-CMP16", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB309446-CMP07", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.
Biological function summary

S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.

Pathways

S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.

S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).
See full target information AHCY
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com