Human SAP ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human SAP ELISA Kit (AB246537)
Example of human SAP standard curve in Sample Diluent NS.
The SAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human SAP ELISA Kit (AB246537)
Interpolated concentrations of native SAP in human serum and plasma samples.
The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.025%, plasma (citrate) 0.025%, plasma (EDTA) 0.025%, and plasma (heparin) 0.025%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 18,069 ng/mL in serum, 15,021 ng/mL in plasma (citrate), 16,223 ng/mL in plasma (EDTA), and 16,400 ng/mL in plasma (heparin).
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제품 세부 정보
Human SAP ELISA Kit (ab246537) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SAP protein in edta plasma, hep plasma, serum, cell culture media, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SAP with 181 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.
Pathways
SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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