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AB303751

Human SEPP1 ELISA Kit

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Human SEPP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEPP1 with a sensitivity of 12.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Interpolated concentrations of human SEPP1 in HepG2 supernatant.

Interpolated concentration of native SEPP1 was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Interpolated concentrations of SEPP1 in normal human serum donors.

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean SEPP1 concentration was determined to be 10.28 μg/mL with a range of 1.41 – 14.21 μg/mL.

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Interpolated concentrations of human SEPP1 in serum, plasma (citrate), plasma (heparin), and plasma (EDTA).

Interpolated concentration of native SEPP1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Example of human SEPP1 standard curve in Sample Diluent NS.

Example of human SEPP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 12.65 pg/mL

검출 범위

31.25 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Primary Antibodies

AB155185

Anti-SEPP1 antibody

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Proteins & Peptides

AB235969

Recombinant Human SEPP1 protein (His tag)

SDS-PAGE - Recombinant Human SEPP1 protein (His tag) (AB235969)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human SEPP1 SimpleStep ELISA® kit (ab303751) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SEPP1 protein in serum, plasma (hep), plasma (cit), plasma (EDTA) and cell culture supernatant. Quantitate Human SEPP1 with 12.65 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 88 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "98 - 124 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "110 - 122 %", "average": "= 117" }, { "sample": "Heparin Plasma", "range": "101 - 112 %", "average": "= 107" }, { "sample": "Serum", "range": "91 - 107 %", "average": "= 98" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SEPP1 also known as Selenoprotein P is a glycoprotein characterized by multiple selenocysteine residues which play a critical role in its function. SEPP1 has a molecular mass of approximately 60 kDa. It expresses predominantly in the liver but can be found in other tissues like the brain kidney and plasma. In the liver it is involved in selenium transport and homeostasis carrying selenium to other tissues through the blood.
Biological function summary

SEPP1 acts as an antioxidant helping to defend cells against damage from reactive oxygen species. It exists as part of a complex system within the body to balance oxidative stress. Moreover SEPP1 contributes to the recycling of selenium in the body ensuring adequate supply for selenoprotein synthesis in various tissues. It serves essential functions in neurological health and immune response leveraging its selenium content to protect cells and maintain cellular activities.

Pathways

SEPP1 is significantly involved in selenium homeostasis and the oxidative stress response pathways. It interacts with proteins like glutathione peroxidase (GPx) and thioredoxin reductase which also depend on selenium for their antioxidant activities. The involvement of SEPP1 in these pathways is critical for mitigating oxidative damage and supporting the function of other selenium-dependent enzymes essential for maintaining cellular health.

SEPP1 is associated with neurodegenerative diseases and certain types of cancer. Dysregulation of SEPP1 and selenium homeostasis may contribute to the development and progression of conditions such as Alzheimer's disease potentially involving interactions with amyloid precursor protein (APP) and tau proteins. In cancer alterations in SEPP1 expression have been linked to colorectal cancer through pathways intersecting with proteins influencing cell proliferation and apoptosis such as Bcl-2 and p53 indicating its potential role in tumor biology.
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제품 프로토콜

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타겟 정보

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
See full target information SELENOP

대체 명칭 보기

SELP, SEPP1, SELENOP, Selenoprotein P, SeP
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websiteProtocolBooklet
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Product promise

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