AB239430

Human Serpin A12 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Human Serpin A12 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Serpin A12 with a sensitivity of 3.12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

Serpin A12, OL-64, Visceral adipose tissue-derived serine protease inhibitor, Visceral adipose-specific serpin, Vaspin, SERPINA12

5 이미지

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

sELISA

Unknown

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 5068.08 pg/mL with a range of undetectable - 9406.87 pg/mL.

sELISA

Unknown

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Interpolated concentrations of native Serpin A12 in human serum, plasma and saliva samples. The concentrations of Serpin A12 were measured in duplicates, interpolated from the Serpin A12 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 15%, plasma (citrate) 50%, plasma (EDTA) 100%, plasma (heparin) 25% and saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 4862.24 pg/mL in serum, 1422.93 pg/mL in plasma (citrate), 1275.97 pg/mL in plasma (EDTA), 4493.91 pg/mL in plasma (heparin), and 208.10 pg/mL in saliva.

sELISA

Unknown

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Human Serpin A12 gamma Standard Curve Comparison. Standard Curve comparison between Human Serpin A12 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Unknown

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 1672.82 pg/mL with a range of undetectable - 6317.42 pg/mL.

sELISA

Unknown

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Example of human Serpin A12 standard curve in Sample Diluent NS. The Serpin A12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Saliva, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3.12 pg/mL

검출 범위

19.53 - 1250 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Human Serpin A12 ELISA Kit (ab239430) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Serpin A12 protein in cit plasma, edta plasma, hep plasma, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Serpin A12 with 3.12 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "103 - 110 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "95 - 102 %", "average": "= 99" }, { "sample": "Saliva", "range": "87 - 105 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "97 - 105 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "81 - 102 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "96 - 100 %", "average": "= 97" } ]

style

left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239430-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239430-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239430-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239430-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239430-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239430-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239430-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB239430-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Serpin A12 Capture Antibody", "number":"AB239430-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Serpin A12 Lyophilized Recombinant Protein", "number":"AB239430-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Serpin A12 Detector Antibody", "number":"AB239430-CMP07", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

style

left-column

style

left-column

제품 프로토콜

style

left-column

타겟 정보

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

See full target information Serpin A12

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Serpin A12 ELISA Kit

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

Sandwich ELISA - Human Serpin A12 ELISA Kit (AB239430)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Secondary Antibodies

Assay Kits

Primary Antibodies

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Product promise