AB326741
Human SynCAM/CADM1 ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- 각 태그의 뜻
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Human SynCAM/CADM1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human SynCAM/CADM1 with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 9.14 - 20000 pg/ml with a sensitivity of 5.72 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 9.14 - 20000 pg/ml with a sensitivity of 5.72 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 이미지
- sELISA
Supplier Data
Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)
Serum of ten individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean SynCAM/CADM1 concentration was determined to be 3022 pg/mL with a range of 1438 – 8126 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)
Interpolated concentration of native SynCAM/CADM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 25% plasma (citrate) 12.5% plasma (EDTA) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Lab
Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)
Dynamic range comparison between the Chemiluminescent Human SynCAM/CADM1 SimpleStep ELISA, colorimetric Human SynCAM/CADM1 SimpleStep ELISA (ab316889), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
- sELISA
Lab
Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (AB326741)
Standard curve comparison between the Chemiluminescent Human SynCAM/CADM1 SimpleStep ELISA and colorimetric Human SynCAM/CADM1 SimpleStep ELISA (ab316889) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
제품 세부 정보
Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (ab326741) is a rapid single-wash sandwich 55-min ELISA to measure Human SynCAM/CADM1 in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma with a sensitivity of 5.72 pg/mL.
How the assay works
Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (ab326741) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human SynCAM/CADM1 (ab326741) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-SynCAM/CADM1 antibody [EPR25965-58] - BSA and Aside free (Capture) ab308309
- Anti-SynCAM/CADM1 antibody [EPR25965-89] - BSA and Aside free (Detector) ab308310
How the assay works
Human SynCAM/CADM1 ELISA Kit, Chemiluminescent (ab326741) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human SynCAM/CADM1 (ab326741) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-SynCAM/CADM1 antibody [EPR25965-58] - BSA and Aside free (Capture) ab308309
- Anti-SynCAM/CADM1 antibody [EPR25965-89] - BSA and Aside free (Detector) ab308310
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" } ]
Recovery
[ { "sample": "Serum", "range": "88 - 93 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "97 - 100 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "90 - 95 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "92 - 100 %", "average": "= 96" } ]
제품 구성
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특성 및 보관 정보
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
타겟 정보
Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner (PubMed : 12050160, PubMed : 22438059). Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner (By similarity). Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed : 15811952). In mast cells, may mediate attachment to and promote communication with nerves (PubMed : 15905536). CADM1, together with MITF, is essential for development and survival of mast cells in vivo (PubMed : 22438059). By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node (By similarity). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (By similarity). Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (By similarity). Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons (By similarity). May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa (By similarity). Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells (PubMed : 11279526, PubMed : 12234973). May contribute to the less invasive phenotypes of lepidic growth tumor cells (PubMed : 12920246).. Isoform 5. (Microbial infection) Induces cell fusion in neuron infected by a neuropathogenic strain of measles. Interacts with measles hemagglutinin to trigger hyperfusogenic F-mediated membrane fusion and presumably transsynaptic cell-to-cell transmission of the virus.
대체 명칭 보기
IGSF4, IGSF4A, NECL2, SYNCAM, TSLC1, CADM1, Cell adhesion molecule 1, Immunoglobulin superfamily member 4, Nectin-like protein 2, Spermatogenic immunoglobulin superfamily, Synaptic cell adhesion molecule, Tumor suppressor in lung cancer 1, IgSF4, NECL-2, SgIgSF, SynCAM, TSLC-1
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