Human Syndecan-1 ELISA Kit (CD138)

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Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.

A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.

This kit will recognize both endogenous and recombinant Human Syndecan-1.

Human Syndecan-1 ELISA Kit (CD138) ab46506 is a sandwich ELISA to measure Human Syndecan (CD138) in serum, plasma, buffered solutions and supernatants. with a sensitivity of 4.94 ng/mL.

How the assay works

A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.

Assay specificity

This kit will recognize both endogenous and recombinant Human Syndecan-1.

Human Syndecan-1 ELISA Kit (CD138) ab46506 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
4. Aspirate and wash each well. Add the TMB Solution to each well until color develops and then add the Stop Solution. Immediately begin recording the color development

How other researchers are using Human Syndecan-1 ELISA Kit (CD138) ab46506

Human Syndecan-1 ELISA Kit (CD138) ab46506 has been used to measure endothelial glycocalyx degradation in children with sepsis ⁽¹⁾ and in traumatic hemorrage ⁽²⁾.

References

⁽¹⁾J Fernandez-Sarmiento et al. 2024, PMID:38245695
⁽²⁾ S. Abdullah et al. 2023, PMID: 37315138

Related and recommended products

Human Syndecan-1 ELISA Kit (CD138) ab46506 is commonly used to study inflammation, endothelial dysfunction and other cellular processes.

Other ELISA kits commonly use alongside Syndecan-1 include:

- Human VEGF ELISA Kit ab222510
- Human IL-6Elisa Kit ab178013
- Human MMP9 ELISA Kit ab246539
- Human ICAM1 ELISA Kit (CD54) ab174445