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AB325909

Human TARC (CCL17) ELISA Kit, Chemiluminescent

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Human TARC (CCL17) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human TARC (CCL17) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.46 - 1000 pg/mL with a sensitivity of 0.3 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Lab

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Lab

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

Pooled healthy and atopic dermatitis serum samples were assayed at various concentrations. The mean interpolated, dilution factor corrected values were : 16.3 pg/mL for healthy serum and 247.1 pg/mL for atopic dermatitis serum.

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Lab

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

Dynamic range comparison between the Chemiluminescent Human TARC SimpleStep ELISA, colorimetric Human TARC SimpleStep ELISA (ab289696), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Lab

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

Standard curve comparison between the Chemiluminescent Human TARC SimpleStep ELISA and colorimetric Human TARC SimpleStep ELISA (ab289696) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Supplier Data

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

Interpolated concentrations of TARC in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration of detected samples was determined to be 7.28 pg/mL with a range of undetected – 12.6 pg/mL.

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Supplier Data

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

Interpolated concentrations of human TARC in cell culture supernatant. Interpolated concentration of native TARC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 20 PBMC treated with 1.5% PHA-M for 46 hours and 1 : 20 PBMC untreated. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)
  • sELISA

Supplier Data

Sandwich ELISA - Human TARC (CCL17) ELISA Kit, Chemiluminescent (AB325909)

Interpolated concentrations of human TARC in serum, plasma - citrate, and plasma - EDTA. Interpolated concentration of native TARC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 100%, plasma - citrate 50%, and plasma - EDTA 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.3 pg/mL

검출 범위

0.46 - 1000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Reagents, Controls & Accessories

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human TARC (CCL17) ELISA Kit, Chemiluminescent (ab325909) is a rapid single-wash sandwich 55-min ELISA to measure Human TARC (CCL17) in Serum, Plasma – Citrate, Plasma – EDTA, Cell Culture Supernatant with a sensitivity of 0.3 pg/mL.

How the assay works

Human TARC (CCL17) ELISA Kit, Chemiluminescent (ab325909) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TARC (CCL17) (ab325909) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TARC antibody [EPR21766-150] - BSA and Aside free (Capture) ab288599
- Anti-TARC antibody [EPR21766-165] - BSA and Aside free (Detector) ab288617
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.7" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 91 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "87 - 91 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "91 - 102 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "89 - 95 %", "average": "= 93" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed : 8702936, PubMed : 9169480). Acts by binding to CCR4 at T-cell surface (PubMed : 10540332, PubMed : 9169480). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed : 27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (By similarity). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (By similarity).
See full target information CCL17

대체 명칭 보기

SCYA17, TARC, CCL17, C-C motif chemokine 17, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine
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websiteProtocolBooklet
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Product promise

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