AB323735

Human Tau (phospho S199) ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human Tau (phospho S199) ELISA Kit- Intracellular is a Sandwich ELISA for the measurement of Human Tau (phospho S199) - Intracellular in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

8,800 pg/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pT217, Tau pT231 and Tau pS396) were tested for specificity and for interference with 4,400 pg/mL of recombinant Tau-A ligated with a peptide containing phosphorylated S199 (Tau pS199). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pS199 control (for interference), are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Example of human Tau (phospho S199) standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Example of human Tau (phospho S199) standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Interpolated concentration of native Tau (phospho S199) was measured in duplicate at different sample concentrations. Undiluted samples are as follows : brain cortex tissue extract 6 µg/mL, brain hippocampus tissue extract 12 µg/mL, and SH-SY5Y cell extract 16 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Interpolated concentrations of native (A) human Tau (phospho S199) and (B) human Tau (total, measured using ab273617) were measured in duplicates at five different sample concentrations of normal and individual Alzheimer’s Disease (AD) human post-mortem brain tissue extract samples. The interpolated dilution factor corrected values, normalized per µg extract, are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Interpolated concentration of native Tau (phospho S199) was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 6 µg/mL brain cortex tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Tau (phospho S199) and Tau (total, measured using Human Tau ELISA Kit ab273617) were measured in duplicate of lambda phosphatase treated (NEB P0753, 96,000 U/mL for 20 min) and mock treated mouse brain tissue extract samples. The raw O.D. values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Interpolated concentrations of (A) Tau (phospho S199) and (B) Tau (total, measured using Human Tau ELISA Kit ab273617) were measured in duplicates at five different sample concentrations of normal human, mouse, and rat brain tissue extract samples. The interpolated values, normalized per µg extract, are plotted (mean +/- SD, n=2).

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 16.3 pg/mL

검출 범위

68.75 - 4400 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Tau (phospho S199) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho S199) protein in Cell Lysate, Tissue extracts. Quantitate Human Tau (phospho S199) with 16.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 14.1" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "105 - 109 %", "average": "= 107" }, { "sample": "Tissue Extracts", "range": "100 - 109 %", "average": "= 104" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho S199

Additional targets

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Mtapt, Tau, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Mtapt, Tau, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Tau (phospho S199) ELISA Kit- Intracellular

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

Sandwich ELISA - Human Tau (phospho S199) ELISA Kit- Intracellular (AB323735)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

Product promise