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AB325894

Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular

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Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Tau (phospho S396) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.12 - 9000 pg/ml with a sensitivity of 2.27 pg/mL.
- Validated on a number of sample types including cerebrospinal fluid (CSF).

- Patent pending
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Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Dynamic range comparison between the Chemiluminescent Human Tau (phospho S396) SimpleStep ELISA, colorimetric Human Tau (phospho S396) SimpleStep ELISA (ab324328), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased range.

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Standard curve comparison between the Chemiluminescent Human Tau (phospho S396) SimpleStep ELISA and colorimetric Human Tau (phospho S396) SimpleStep ELISA (ab324328) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Lab

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tau (phospho S396) concentration of detected samples was determined to be 125.5 pg/mL with a range of undetected – 125.5 pg/mL.

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Interpolated concentrations of native (A) human Tau (phospho S396) of 6 individual post-mortem human CSF donors measured in singlet and (B) human Tau (total, measured using ab273617) of the same 6 individual post-mortem human CSF donors measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted.

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pT217, Tau pT231 and Tau pS199) were tested for specificity (each at 25,600 pg/mL), and for interference (each at 12,800 pg/mL) combined with 1,600 pg/mL of recombinant Tau-A ligated with a peptide containing phosphorylated S396 (Tau pS396). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pS396 control (for interference), are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)
  • sELISA

Supplier Data

Sandwich ELISA - Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (AB325894)

Interpolated concentration of native Tau (phospho S396) was measured in duplicate at different sample concentrations. Undiluted samples are 50% pooled post-mortem CSF. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.27 pg/mL

검출 범위

4.12 - 9000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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AB318936

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  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (ab325894) is a rapid single-wash sandwich 55-min ELISA to measure Human Tau (phospho S396) in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant, Cerebrospinal Fluid with a sensitivity of 2.27 pg/mL.

How the assay works

Human Tau (phospho S396) ELISA Kit, Chemiluminescent - Extracellular (ab325894) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Tau (phospho S396) (ab325894) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.1" } ]
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Recovery

[ { "sample": "Serum", "range": "78 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "90 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "82 - 89 %", "average": "= 87" }, { "sample": "Cell culture supernatant", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Cerebral Spinal Fluid", "range": "87 - 95 %", "average": "= 90" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT pS396

대체 명칭 보기

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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