Human Tau (phospho S396) ELISA Kit- Extracellular
- SimpleStep
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- sELISA
Supplier Data
Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)
Interpolated concentrations of native (A) human Tau (phospho S396) of 6 individual post-mortem human CSF donors measured in singlet and (B) human Tau (total, measured using ab273617) of the same 6 individual post-mortem human CSF donors measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted.
- sELISA
Supplier Data
Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)
Recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT181, Tau pT217, Tau pT231 and Tau pS199) were tested for specificity (each at 25,600 pg/mL), and for interference (each at 12,800 pg/mL) combined with 1,600 pg/mL of recombinant Tau-A ligated with a peptide containing phosphorylated S396 (Tau pS396). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pS396 control (for interference), are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)
Interpolated concentration of native Tau (phospho S396) was measured in duplicate at different sample concentrations. Undiluted samples are 50% pooled post-mortem CSF. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)
Example of human Tau (phospho S396) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Tau (phospho S396) ELISA Kit- Extracellular (AB324328)
Example of human Tau (phospho S396) standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Human Tau (phospho S396) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau (phospho S396) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Cerebral Spinal Fluid. Quantitate Human Tau (phospho S396) with 20.957 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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