Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular
- Recombinant
- SimpleStep Ignite™ ELISA
- 각 태그의 뜻
Be the first to review this product! 리뷰 제출
|
(0 출판물)
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.12 - 9000 pg/ml with a sensitivity of 1.06 pg/mL.
- Validated on a number of sample types including cerebrospinal fluid (CSF).
- Patent pending
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
Interpolated concentrations of native (A) human Tau (phospho T181) and (B) human Tau (total, measured using Human Tau ELISA Kit ab273617) of 6 individual post-mortem human CSF donors were measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tau (phospho T181) concentration of detected samples was determined to be 405 pg/mL with a range of undetected - 679 pg/mL.
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
Dynamic range comparison between the Chemiluminescent Human Tau (phospho T181) SimpleStep ELISA, colorimetric Human Tau (phospho T181) SimpleStep ELISA (ab325078), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and range.
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
Standard curve comparison between the Chemiluminescent Human Tau (phospho T181) SimpleStep ELISA and colorimetric Human Tau (phospho T181) SimpleStep ELISA (ab325078) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
Serum of ten individual healthy human female donors was measured in duplicate using the colorimetric version of this kit (ab325078). Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tau (phospho T181) concentration of detected samples was determined to be 904 pg/mL with a range of undetected – 904 pg/mL. The nine donor sera with undetected values using the ab325078 had also undetected values using this kit.
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
Interpolated concentration of native Tau (phospho T181) was measured in duplicate at different sample concentrations. Undiluted samples are as follows : pooled post-mortem cerebrospinal fluid 100% and an individual female healthy serum 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Lab
Sandwich ELISA - Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (AB325895)
Human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid were tested for specificity and interference. 48,000 pg/mL of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (non-phosphorylated Tau, Tau pT217, Tau pT231, Tau pS199, and Tau pS396) were tested for specificity. Different concentrations of recombinant human Tau-A proteins each ligated with a peptide containing a specific phosphorylated amino acid (24,000 pg/mL : non-phosphorylated Tau, Tau pT231, Tau pS199, and Tau pS396; 6,000 pg/mL : Tau pT217) were tested for interference with 1,500 pg/mL of recombinant Tau-A ligated with a peptide containing phosphorylated T181 (Tau pT181). The interpolated dilution-corrected values, expressed in % of input (for specificity) and in % of Tau pT181 control (for interference), are plotted (mean +/- SD, n=2).
보완 제품
Reactivity 정보
제품 세부 정보
How the assay works
Human Tau (phospho T181) ELISA Kit, Chemiluminescent - Extracellular (ab325895) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Tau (phospho T181) (ab325895) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Precision
Recovery
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
타겟 정보
대체 명칭 보기
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com