AB242251

Human TECK ELISA Kit

Recombinant
SimpleStep
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Human TECK ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TECK with a sensitivity of 1.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

2 이미지

Sandwich ELISA - Human TECK ELISA Kit (AB242251)

sELISA

Unknown

Sandwich ELISA - Human TECK ELISA Kit (AB242251)

Interpolated concentrations of spiked TECK in human serum, plasma and cell culture supernatant samples. The concentrations of TECK were measured in duplicates, interpolated from the TECK standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 12.5%, and cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TECK concentration was determined to be 1007.84 pg/mL in serum, 1066.68 pg/mL in plasma (citrate), 1091.29 pg/mL in plasma (EDTA) and 916.63 pg/mL in cell culture supernatant.

sELISA

Unknown

Sandwich ELISA - Human TECK ELISA Kit (AB242251)

Example of human TECK standard curve in Sample Diluent NS. The TECK standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.9 pg/mL

검출 범위

23.44 - 1500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human TECK ELISA Kit (ab242251) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TECK protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TECK with 1.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 100 %", "average": "= 99" }, { "sample": "Serum", "range": "91 - 99 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "93 - 100 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 99 %", "average": "= 97" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CCL25 also known as TECK stands as a chemokine with a central role in immune cell attraction. It weighs approximately 15 kDa and is mainly expressed in the thymus and small intestine. This chemokine's expression is associated with its activity in guiding the movement of various immune cells like lymphocytes. Additionally CCL25 interacts with its receptor CCR9 facilitating important cellular signaling necessary for immune responses.

Biological function summary

This chemokine aids in the migration and homing of T-cell subsets particularly to the gut and small intestine hinting at its role in mucosal immunity. CCL25 does not belong to a larger multiprotein complex but it plays a standalone role in controlling immune surveillance and response. Its involvement contributes to the precise regulation of immune cell localization especially under inflammatory conditions.

Pathways

CCL25 participates significantly in the chemokine signaling pathway and the integrin-mediated adhesion pathway. It cooperates with proteins like CCR9 which is its receptor to activate downstream signaling cascades necessary for immune cell trafficking and adhesion. Through these pathways CCL25 ensures correct immune cell distribution throughout the body supporting effective immune surveillance and response.

CCL25 is associated with inflammatory bowel disease (IBD) and celiac disease. In these conditions CCL25 together with its receptor CCR9 contributes to the recruitment of lymphocytes to the inflamed gut. This recruitment exacerbates inflammation and may also link to other inflammatory proteins like TNF-alpha fostering an environment of sustained inflammation. Understanding CCL25's role in such diseases could inform therapeutic strategies aimed at modulating immune cell trafficking.

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제품 프로토콜

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타겟 정보

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

See full target information CCL25

대체 명칭 보기

SCYA25, TECK, CCL25, C-C motif chemokine 25, Chemokine TECK, Small-inducible cytokine A25, Thymus-expressed chemokine

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Human TECK ELISA Kit

Sandwich ELISA - Human TECK ELISA Kit (AB242251)

Sandwich ELISA - Human TECK ELISA Kit (AB242251)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise