Human Tetranectin ELISA Kit (CLEC3B)

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The Human Tetranectin Enzyme-Linked Immunosorbent Assay (ELISA) kit (CLEC3B) (ab213832) is designed for the quantitative measurement of Human Tetranectin in cell supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for Tetranectin/CLEC3B has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E22-V202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for Tetranectin/CLEC3B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tetranectin/CLEC3B amount of sample captured in plate.

Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and non-covalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.