AB327324
Human TFPI ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- 각 태그의 뜻
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Human TFPI ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human TFPI with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.66 - 8000 pg/ml with a sensitivity of 1.13 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.66 - 8000 pg/ml with a sensitivity of 1.13 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
5 이미지
- sELISA
Supplier Data
Sandwich ELISA - Human TFPI ELISA Kit, Chemiluminescent (AB327324)
Interpolated concentrations of human TFPI in human serum plasma (citrate) plasma (EDTA) and plasma (heparin)
- sELISA
Supplier Data
Sandwich ELISA - Human TFPI ELISA Kit, Chemiluminescent (AB327324)
Interpolated concentrations of human TFPI in human urine and HepG2 cell culture supernatant
- sELISA
Supplier Data
Sandwich ELISA - Human TFPI ELISA Kit, Chemiluminescent (AB327324)
Interpolated concentrations of TFPI in normal human serum donors
- sELISA
Lab
Sandwich ELISA - Human TFPI ELISA Kit, Chemiluminescent (AB327324)
Standard curve comparison between the Chemiluminescent Human TFPI SimpleStep ELISA and colorimetric Human TFPI SimpleStep ELISA (ab274392) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human TFPI ELISA Kit, Chemiluminescent (AB327324)
Dynamic range comparison between the Chemiluminescent Human TFPI SimpleStep ELISA, colorimetric Human TFPI SimpleStep ELISA (ab274392), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
제품 세부 정보
Human TFPI ELISA Kit, Chemiluminescent (ab327324) is a rapid single-wash sandwich 55-min ELISA to measure Human TFPI in Cell culture supernatant, Serum, EDTA Plasma, Urine, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 1.13 pg/mL.
How the assay works
Human TFPI ELISA Kit, Chemiluminescent (ab327324) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human TFPI (ab327324) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-TFPI antibody [EPR22977-118] - BSA and Aside free (Capture) ab274436
- Anti-TFPI antibody [EPR22977-124] - BSA and Aside free (Detector) ab274437
How the assay works
Human TFPI ELISA Kit, Chemiluminescent (ab327324) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human TFPI (ab327324) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-TFPI antibody [EPR22977-118] - BSA and Aside free (Capture) ab274436
- Anti-TFPI antibody [EPR22977-124] - BSA and Aside free (Detector) ab274437
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]
Recovery
[ { "sample": "Cell culture supernatant", "range": "110 - 112 %", "average": "= 106" }, { "sample": "Serum", "range": "111 - 119 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "114 - 117 %", "average": "= 116" }, { "sample": "Urine", "range": "105 - 117 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "88 - 94 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "113 - 120 %", "average": "= 117" }, { "sample": "Citrate plasma", "range": "96 - 106 %", "average": "= 102" } ]
제품 구성
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특성 및 보관 정보
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
타겟 정보
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
대체 명칭 보기
LACI, TFPI1, TFPI, Tissue factor pathway inhibitor, Extrinsic pathway inhibitor, Lipoprotein-associated coagulation inhibitor, EPI
Product promise
저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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