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AB100646

Human TGF alpha ELISA Kit

5

(1 리뷰)

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(4 제품이 사용된 논문 )

Human TGF alpha ELISA Kit is a Sandwich ELISA for the measurement of Human TGF alpha in Human in Biofluids, Cell Culture Media samples.

대체 명칭 보기

Protransforming growth factor alpha, TGFA

2 이미지
Sandwich ELISA - Human TGF alpha ELISA Kit (AB100646)
  • sELISA

Unknown

Sandwich ELISA - Human TGF alpha ELISA Kit (AB100646)

Representative standard curve using ab100646

Sandwich ELISA - Human TGF alpha ELISA Kit (AB100646)
  • sELISA

Unknown

Sandwich ELISA - Human TGF alpha ELISA Kit (AB100646)

Representative standard curve using ab100646

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 3 pg/mL

검출 범위

1.37 - 1000 pg/mL

분석 플랫폼

Microplate

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's TGF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF alpha in serum, plasma, cell culture supernatants

This assay employs an antibody specific for Human TGF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 105 %", "average": "= 94.49" }, { "sample": "Serum", "range": "82 - 104 %", "average": "= 92.34" }, { "sample": "Plasma", "range": "83 - 103 %", "average": "= 93.46" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100646-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100646-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100646-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100646-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TGF alpha Microplate (12 x 8 wells)", "number":"AB100646-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100646-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100646-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human TGF alpha Standard (lyophilized)", "number":"AB100646-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human TGF alpha", "number":"AB100646-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100646-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
See full target information Protransforming growth factor alpha
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

Oncoimmunology 5:e1115177 PubMed27141367

2016

G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer.

Applications

Unspecified application

Species

Human

Maija Hollmén,Sinem Karaman,Simon Schwager,Angela Lisibach,Ailsa J Christiansen,Mikael Maksimow,Zsuzsanna Varga,Sirpa Jalkanen,Michael Detmar

Prostaglandins, leukotrienes, and essential fatty acids 90:105-115 PubMed24374147

2014

Key roles for GRB2-associated-binding protein 1, phosphatidylinositol-3-kinase, cyclooxygenase 2, prostaglandin E2 and transforming growth factor alpha in linoleic acid-induced upregulation of lung and breast cancer cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

M Mouradian,K D Kikawa,E D Johnson,K L Beck,R S Pardini

PloS one 8:e70496 PubMed23936217

2013

Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Tukaj,Konrad Kleszczyński,Katerina Vafia,Stephanie Groth,Damian Meyersburg,Piotr Trzonkowski,Ralf J Ludwig,Detlef Zillikens,Enno Schmidt,Tobias W Fischer,Michael Kasperkiewicz
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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