AB289642

Human Thyroxine Binding Globulin ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Human Thyroxine Binding Globulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thyroxine Binding Globulin with a sensitivity of 8.624 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

TBG, SERPINA7, Thyroxine-binding globulin, Serpin A7, T4-binding globulin

3 이미지

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

sELISA

Supplier Data

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Interpolated concentrations of human Thyroxine Binding Globulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Thyroxine Binding Globulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 100, plasma (EDTA) 1 : 100, and plasma (heparin) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Interpolated concentrations of spiked human Thyroxine Binding Globulin in cell culture media (10F RPMI).

Recombinant Thyroxine Binding Globulin was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% cell culture media (10F RPMI). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Example of human Thyroxine Binding Globulin standard curve in Sample Diluent NS.

Example of human Thyroxine Binding Globulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8.624 pg/mL

검출 범위

18.75 - 1200 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Thyroxine Binding Globulin ELISA Kit (ab289642) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Thyroxine Binding Globulin in serum, plasma (heparin), plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thyroxine Binding Globulin with 8.624 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]

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Recovery

[ { "sample": "Serum", "range": "81 - 113 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "107 - 113 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "112 - 117 %", "average": "= 115" }, { "sample": "Heparin Plasma", "range": "93 - 116 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "87 - 96 %", "average": "= 91" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289642-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289642-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB289642-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289642-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289642-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289642-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289642-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289642-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thyroxine Binding Globulin Capture Antibody", "number":"AB289642-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Thyroxine Binding Globulin Lyophilized Recombinant Protein", "number":"AB289642-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Thyroxine Binding Globulin Detector Antibody", "number":"AB289642-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.

Biological function summary

The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.

Pathways

Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.

The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.

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제품 프로토콜

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타겟 정보

Major thyroid hormone transport protein in serum.

See full target information SERPINA7

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Heliyon 9:e21616 PubMed38027820

2023

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan Zhang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Thyroxine Binding Globulin ELISA Kit

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

Sandwich ELISA - Human Thyroxine Binding Globulin ELISA Kit (AB289642)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise