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AB187394

Human TIMP1 ELISA Kit

Human TIMP1 ELISA 키트

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(11 제품이 사용된 논문 )

Human TIMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TIMP1 with a sensitivity of 17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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ELISA - Human TIMP1 ELISA Kit (AB187394)
  • ELISA

Supplier Data

ELISA - Human TIMP1 ELISA Kit (AB187394)
Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Lab

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of human TIMP-1 in serum in 96-well vs. 384-well plates

Interpolated concentration of native TIMP-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Lab

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Example of human TIMP-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 92.6 ng/mL with a range of 66.5 – 128.7 ng/mL.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Human TIMP-1 standard curve comparison.

Standard Curve comparison between the original Human TIMP-1 SimpleStep ELISA kit and current Human TIMP-1 SimpleStep ELISA kit.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of native TIMP-1 in human cell culture supernatant samples.

The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMC cell culture supernatant 20% and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 5 ng/mL in unstimulated and 22 ng/mL in stimulated and undetectable in media.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Human TIMP-1 competitor curve comparison.

Standard Curve comparison between Human TIMP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Example of human TIMP-1 standard curve in Sample Diluent NS.

The TIMP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of native TIMP-1 in human saliva sample.

The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 2%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 179.4 ng/mL in saliva.

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)
  • sELISA

Supplier Data

Sandwich ELISA - Human TIMP1 ELISA Kit (AB187394)

Interpolated concentrations of native TIMP-1 in human serum, and plasma samples.

The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 95.9 ng/mL in serum, 74 ng/mL in plasma (citrate), 68 ng/mL in plasma (EDTA) and 56 ng/mL in plasma (heparin).

주요 정보

검출 방식

Colorimetric

샘플 타입

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 17 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Microplate

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Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human TIMP1 ELISA Kit ab187394 is a rapid single-wash 90-min Sandwich ELISA to measure Human TIMP1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum. This SimpleStep sensitivity is 17 pg/mL.

How the assay works

Human TIMP1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TIMP1 ELISA Kit ab187394 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human TIMP1 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TIMP1 antibody [EPR21887-129] - BSA and Azide free (Capture) ab244629
- Anti-TIMP1 antibody [EPR21887-34] - BSA and Azide free (Detector) ab244890

Tissue inhibitor of metalloproteinases 1 (TIMP1 or Metalloproteinase inhibitor 1) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding non-covalently to MMPs (in a 1:1 stoichiometry) and blocking access of substrate to the MMP active site. TIMP1 expression is induced by pro-inflammatory cytokines. It is a soluble factor found circulating in serum and plasma. TIMP1 appears also have functions that are independent of MMP inhibition, including promoting erythropoiesis and inhibiting apoptosis in B cells.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 92 %", "average": "= 89" }, { "sample": "Serum", "range": "89 - 90 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "86 - 91 %", "average": "= 89" }, { "sample": "Saliva", "range": "82 - 87 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "95 - 99 %", "average": "= 97" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
Biological function summary

TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.

Pathways

TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.

TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
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제품 프로토콜

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타겟 정보

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
See full target information TIMP1

대체 명칭 보기

CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1
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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록and refine your search

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Erotides Capistrano da Silva,Daniel J Gibson,Sunyoung Jeong,Kelli L Zimmerman,Kathryn M Smith-Fleming,Bianca da C Martins

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