AB46087

Human TNF alpha ELISA Kit

Name: Human TNF alpha ELISA Kit
Brand: Abcam Limited
SKU: AB46087
Rating: 5 (1 reviews)

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(39 제품이 사용된 논문 )

Human TNF alpha ELISA Kit is a Sandwich ELISA for the measurement of Human TNF alpha in Human in Biofluids, Cell Culture Media samples.

2 이미지

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

TNFα was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 μg x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) with LPS and control and stimulated PBMCs (PHA 10 μg x mL-1; 3 days). Results are shown with background signal subtracted (duplicates +/- SD), with the positive control from ab46087 included.

sELISA

Unknown

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

Representative standard curve using ab46087

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8 pg/mL

검출 범위

25 - 800 pg/mL

분석 소요 시간

3h 45m

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human TNF alpha ELISA kit (ab46087) is a quantitative ELISA assay for the measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.

A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

This kit will recognize both endogenous and recombinant Human TNF alpha.

Get results in 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA^® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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Recovery

[ { "sample": "Serum", "range": "74 - 90 %", "average": "= 81" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46087-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"TNF alpha Control", "number":"AB46087-CMP05" }, { "size":"2 x 1 Vial", "name":"TNF alpha Standard", "number":"AB46087-CMP06" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46087-CMP10" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46087-CMP13" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46087-CMP09" }, { "size":"1 x 2 Unit", "name":"Plastic Plate Covers", "number":"AB46087-CMP12" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46087-CMP01" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46087-CMP11" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46087-CMP02" }, { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46087-CMP03" }, { "size":"1 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 well strips)", "number":"AB46087-CMP08" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46087-CMP07" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-TNF alpha", "number":"AB46087-CMP04" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46087-2x96Tests", "assetComponentDetails": [ { "size":"4 x 1 Vial", "name":"TNF alpha Control", "number":"AB46087-CMP05", "productcode":"" }, { "size":"4 x 1 Vial", "name":"TNF alpha Standard", "number":"AB46087-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"TNF alpha Microplate (12 x 8 well strips)", "number":"AB46087-CMP08", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46087-CMP09", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46087-CMP13", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46087-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46087-CMP10", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46087-CMP01", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46087-CMP03", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plastic Plate Covers", "number":"AB46087-CMP12", "productcode":"" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46087-CMP11", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46087-CMP07", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-TNF alpha", "number":"AB46087-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed : 16829952, PubMed : 22517918, PubMed : 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed : 12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information TNF

대체 명칭 보기

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

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제품이 사용된 논문 (39)

Recent publications for all applications. Explore the 전체 목록 and refine your search

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IL-6 Affects Liver Metabolic Abnormalities Caused by Silicon Exposure by Regulating the PKC/YY1 Signaling Pathway.

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Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.

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제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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For licensing inquiries, please contact partnerships@abcam.com

Human TNF alpha ELISA Kit

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

Sandwich ELISA - Human TNF alpha ELISA Kit (AB46087)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (39)

Product promise