Human TNFRSF17 ELISA Kit (BCMA)

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The Human TNFRSF17 Enzyme-Linked Immunosorbent Assay (ELISA) kit (BCMA) (ab213840) is designed for the quantitative measurement of Human TNFRSF17 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF17/BCMA has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: M1-A54) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17/BCMA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFRSF17/BCMA amount of sample captured in plate.

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.