Human TNFSF4 ELISA Kit (OX40L)

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The Human TNFSF4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (OX40L) (ab213842) is designed for the quantitative measurement of Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF4/OX40L has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q51-L183) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF4/OX40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFSF4/OX40L amount of sample captured in plate.

OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.