AB324319

Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular

SimpleStep
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Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular is a Sandwich ELISA for the measurement of Human Topoisomerase II beta/TOP2B - Intracellular in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

sELISA

Supplier Data

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Example of human Topoisomerase II beta/TOP2B standard curve in 1X Cell Extraction Buffer SSW + Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Interpolated concentration of native Topoisomerase II beta/TOP2B was measured in duplicate at different sample concentrations. Undiluted samples are as follows : MCF7 cell extract 1,000 µg/mL, THP-1 cell extract 500 µg/mL, and stomach tissue extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW + Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Interpolated concentration of native Topoisomerase II beta/TOP2B was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,000 µg/mL MCF7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW + Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Example of human Topoisomerase II beta/TOP2B standard curve in 1X Cell Extraction Buffer SSW + Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 44.871 pg/mL

검출 범위

250 - 16000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Topoisomerase II beta/TOP2B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Topoisomerase II beta/TOP2B protein in Cell Lysate, Tissue extracts. Quantitate Human Topoisomerase II beta/TOP2B with 44.871 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "90 - 99 %", "average": "= 94" }, { "sample": "Tissue Extracts", "range": "80 - 96 %", "average": "= 87" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation.

See full target information DNA topoisomerase 2-beta

대체 명칭 보기

DNA topoisomerase 2-beta, TOP2B

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

Sandwich ELISA - Human Topoisomerase II beta/TOP2B ELISA Kit- Intracellular (AB324319)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise