Human TRAIL R2 ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
Interpolated concentrations of native TRAIL R2 in human pancreas and colon cancer extract samples.
The concentrations of TRAIL R2 were measured in duplicate, interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are both 500 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
Interpolated concentrations of spiked TRAIL R2 in human serum and plasma samples.
The concentrations of TRAIL R2 were measured in duplicate and interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are follows : serum 50% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
Interpolated concentrations of native TRAIL R2 in human A549, MDA-MB-231, HepG2, and HT1080 cell extract samples.
The concentrations of TRAIL R2 were measured in duplicates, interpolated from the TRAIL R2 standard curves and corrected for sample dilution. Undiluted samples are as follows : A549 extract 250 μg/mL, MDA-MB-231 extract 62.5 μg/mL, HepG2 extract 62.5 μg/mL, and HT1080 extract 31.25 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
Example of human TRAIL R2 standard curve in Sample Diluent NS.
The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
Example of human TRAIL R2 standard curve in 1X Cell Extraction Buffer PTR.
The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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제품 세부 정보
Human TRAIL R2 ELISA Kit (ab233614) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TRAIL R2 protein in edta plasma, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TRAIL R2 with 10.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.
Pathways
DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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